Correspondence to Author:  Ruyun Wang^1,2#, Yunhao Cui^1,3#, Shihui Cao^1,2, Zizhen Ming^4*, Shengtai Bian^1*  

1. Microfluidics Research & Innovation Laboratory, School of Sport Science, Beijing Sport University, Beijing 100084, China
2. School of Sport Medicine and Physical Therapy, Beijing Sport University, Beijing 100084, China
3. School of Sports Engineering, Beijing Sport University, Beijing 100084, China
4. Shanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China

Abstract:

Endurance training has great benefits for health and sports performances. However, the genes involved in endurance training and the trigged metabolic activities are still unclear. In this study, we provide basic insights into potential mechanisms of endurance training and clarify its regulatory effects on myosin and energy metabolism pathway by analyzing differentially expressed genes (DEGs). After analyzing the GSE242354 in the Gene Expression Omnibus (GEO) through bioinformatics techniques, 1325 DEGs were identified, including 200 up-regulated genes and 1125 down-regulated genes. Gene Ontology (G0) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis manifested that DEGs were mainly targeted at organic acid catabolic processes, etc. PPI network was constructed and explored in depth. Three important subnetworks and 14 hub differential genes such as GRWD1 were screened. Their crucial participation in RNA binding, preribosome and other processes were determined in enrichment analysis. The study of gastrocnemius muscle samples revealed that the expressions of MYH7B and MYH10 encoding actin heavy chain and MYL4 encoding light chain increased, indicating that endurance training could induce the enhanced expression of slow muscle fiber phenotype. In addition, FBP2, ALDOC and ENO1 regulated the glycolytic metabolic pathways and made exercises more energy-saving. The expression of ALDH6A1 was up-regulated after training, which affected the synthesis of long-chain fatty acids. This study identified some advisable mechanisms of endurance training and explained them to some extent. In addition, after long-term endurance training, energy metabolism will tend to be energy-saving and efficient in order to produce adaptation.

Citation:

Zizhen Ming,The potential mechanisms and regulation of myosin and energy metabolism in endurance training. Journal of Immunology 2024.