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Correspondence to Author:  Konstantin Galichanin¹, Zhaohua Yu¹, 

Gullstrand lab, Section of Ophthalmology, Dept. of Surgical sciences, Uppsala University, Uppsala, Sweden

DOI: 10.52338/joed.2024.4227

Abstract:
Purposes: The aim of this study is to investigate the time evolution of caspase 3 mRNA expression within first 24 hours in the rat lens after in vivo exposure to subthreshold dose of UVR-B.
Methods:Twelve six-week-old female albino SpragueDawley rats were exposed to subthreshold dose (1 kJ/m2) of UVR-B unilaterally and sacrificed at 1, 8, 16 and 24 hours after exposure. Lenses were enucleated and caspase 3 mRNA expression was detected by qRT-PCR. The time evolution of caspase 3 mRNA expression was then plotted as a function of mean ratios in caspase 3 mRNA expression between exposed and contralateral nonexposed lenses.
Results: There is no difference in mean ratios in caspase 3 mRNA expression between exposed and nonexposed lenses in all four postexposure groups.
Conclusions: Exposure to subthreshold dose of UVR-B does not induce increase in caspase 3 mRNA expression in the rat lens in vivo within first 24 hours.
Translational relevance: Preclinical animal model study of ultraviolet radiation B cataract might lead to the development of new non-surgical therapy of cataract.

Citation:

Dr.Konstantin Galichanin. Exposure To Subthreshold Dose Of Uvr-B Does Not Induce Increase In Caspase 3 Mrna Expression In The Rat Lens In Vivo Within First 24 Hours. Journal of Ophthalmology and Eye Disorders 2024.