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Correspondence to Author: Quelie Selakong Nzekuie¹, Sylvin Benjamin Ateba¹,Noël Babayana. Etaga¹,Christian Tenezogang Takoukam¹, Joël Nguemo Tetio¹,Bienvenu Bongue²,Samuel Honoré Mandengue¹,Dieudonné Massoma Lembe¹,Clarisse Noël Ayina ¹. 

¹ Department of Animal Sciences, Faculty of Sciences, University of Douala, P.O Box: 24157, Douala, Cameroon.
² Sainbiose Inserm U1059 Laboratory, Jean Monnet University, Saint-Etienne, France;

DOI: 10.52338/jotr.2025.4390

Abstract:

Elaeis guineensis roots have been traditionally used for the treatment reproductive troubles. This study aimed to investigate the reproductive toxicity of the aqueous extract of Elaeis guineensis’ roots. Twentyfour males and 48 females (Two subgroups of 24each) aged 9 and 12 weeks were randomly separated into 4 groups of 6 rats each. The control received distilled water (1 mL/po), while the test groups received aqueous extract of E. guineensis at 100, 200 and 400 mg/kg doses per gavage during 54 days (males) and 10 days (females). Subsequently, males were mated in rapport 1:2 (females untreated and treated) during 10 days, while receiving extract. After mating, sexual behavioral parameters (mount latency and frequency, intromission latency and frequency, ejaculation latency and frequency, post ejaculation interval, Average interval of copulation) were recorded in males using ovariectomized female’s rats (10). In females the percentages of fertility, fecundity and prolificacy were calculated. Consecutively, treated rats were serially sacrificed; Sperm count and histologic analysis of androgen and estrogen dependent organs and pituitary gland were evaluated. Organs section of treated groups presented normal architecture, and sexual behavioral parameters did not show any significant difference when compared to control group. Sperm count significantly increased at 200 mg/ kg dose. The percentage of fertility was 100% in all groups, but the percentages of fecundity and prolificacy significantly decreased at the dose of 400 mg/ kg. Aqueous extract of E. guineensis roots did not affect sexual behabior, improved fertility at traditional use dose, but could negatively affect spermatozoids’ structure and fetus development at high doses.

Keywords: Eleais guineensis roots, male sexual behaviour, Male fertility parameters, Offspring, Reproductive toxicity.

Citation:

Dr.Clarisse Noël Ayina ,Reproductive Toxicity Associated To Elaeis Guineensis Roots Extract In Wistar Rat. Journal of Toxicological Research 2025.