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Correspondence to Author: Samantha R. Weaver, 

Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, USA.

INTRODUCTION: 
The concentration of PTH(7–34) utilized in vivo was mentioned wrongly in the figure legends of the Weaver et al. (1) publication, according to a reader of the Journal of Bone and Mineral Research (JBMR®). The Supplemental Figures 2, 5, and 6 as well as the legends to Figures 3–5 contain the mistakes. Correct concentration is 100 μg/kg body weight per day, not 100 mg/kg/day, as stated in the Materials and Methods section. The authors regret these mistakes and have updated the figure legends below. Figure 3 In Phlpp1/ mice, daily PTH(7–34) treatment reverses short limb length. Femur lengths (B,D) and tibia (A,C) in 4-week-old male WT or Phlpp1/U mice were measured daily doses of vehicle (0.1% BSA in PBS) or PTH(7–34) (100 μg/kg body weight/day). At 4 weeks of age, the tibia (E,G) and femur (F,H) lengths of female WT and Phlpp1// mice were measured after receiving the same treatments. 5 mm is the scale bar. Tukey’s post-hoc test was used in conjunction with a two-way ANOVA to identify statistically significant differences. Figure 4: Phlpp1 and Pth1r work together to control the development of growth plates. (A,B) 3D models of the proximal tibial growth plates were created using μCT scans of male 4-week-old WT or Phlpp1/} mice that received daily injections of either vehicle or PTH(7–34) at a dose of 100 μg/kg body weight. The 2D capture’s scale bar is 1 mm. The color scale bar measures millimeters. For e, one sample 3D rendering is displayed ach group. (C) The proximal tibia growth plate’s proliferative and hypertrophic zones were measured. (D) The proximal tibial growth plate was stained with safranin O/fast green. 50 μm is the scale bar. Either the proliferative zone (P) or the hypertrophic zone (H) is indicated by vertical black lines. (E) For Col10a1, in situ hybridization was carried out. 50 μm is the scale bar. (F,G) From postnatal day 1 (P1) to P5, WT or Phlpp1 / mice were injected daily with either vehicle or PTH(7–34), as previously mentioned. BrdU was given to P5 two hours before the procedure. (F) As shown in G, the percentage of BrdU-positive cells in the proximal tibial growth plate was measured. G scale bar: 50 μm. Tukey’s post hoc test was used in conjunction with a two-way ANOVA to identify statistically significant differences.

Citation:

Samantha R. Weaver. Leucine rich repeat phosphatase 1 (Phlpp1) and the pleckstrin homology (PH) domain inhibit parathyroid hormone receptor 1 (Pth1r) production and signaling during bone development. The Journal of Bone and Mineral Research 2024.