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Correspondence to Author: Rawan Hazem Abdelellah^*, Basmala Mahmoud Kamel ,Sara Ibrahim Ibrahim, Hadeer, K.S. Abdelrahman. 

Department of Biochemistry, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza 12451, Egypt.

DOI: 10.52338/job.2025.4362

Abstract:

It was shown that the number of protein-based drugs has been increasing recently, in contrast to synthetic systems, drug delivery methods based on peptides possess numerous advantages because of their superior biocompatibility, biophysical and biochemical characteristics, and absence of toxicity. However, the absence of effective cytosolic delivery methods hinders the development of protein therapies against intracellular targets, where proteins can’t directly flow through the cell membrane by passive diffusion because of their hydrophilic nature. As a result, delivery pathways that use endosomal escape after endocytic absorption have been investigated. As an alternative, delivery strategies that use modified peptides to promote endocytic uptake and endosomal escape or temporary permeabilization of cell membranes have been documented recently. This report emphasized the obstacles of peptide cytosolic delivery. Additionally, a brief overview of the tactics used to facilitate peptide-mediated cytosolic delivery of proteins was given. These tactics included drug delivery systems based on peptides, which are used to transport therapeutic substances like anti-cancer medications and drugs based on nucleic acids. These DDSs not only slow down the rate at which drugs degrade, but also guarantee the amount of the drug at the desired site and extend the t 1/2 of drugs in vivo. Lastly, the most popular in vitro methods for figuring out how protein cargo is distributed inside cells were described.

Keywords:Intracellular targets, Cell-penetrating peptides, Cytosolic protein delivery, internalization, endosomal escape

Citation:

Dr.Rawan Hazem Abdelellah ,Different Peptide Cytosolic Delivery Methods. Journal of Biochemistry 2025.