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Correspondence to Author: Ramazan ali Dianati tilaki^*1, Masoumeh Eslamifar²,Kobra Zabihzadeh³. 

Master Student-Chemical Engineering Department- Sharif University of Technology, Tehran, Iran.

DOI: 10.52338/joes.2025.4387

Abstract:

Physico-chemical methods for removing low concentration dispersed Total Petroleum Hydrocarbons (TPH) from water are not cost-effective. The aim of this study was to remove TPH from water by combined cultivation of Azolla and bacteria. Concentrations of petroleum hydrocarbon in culture medium were 100,500 and 1000 mgL-1. Study was conducted over 14 days in three modes by cultivation of Azolla, bacteria and combined Azolla-bacteria culture. Concentration of Petroleum hydrocarbon (TPH) in the water samples was measured using gas chromatography. For cultivation of Azolla, the TPH removal from water after 14 days of contact was 76%, 70%, and 62% for initial TPH concentrations of 100, 500, and 1000 mgL-1 respectively. For bacterial cultivation, for an initial TPH concentration of 1000 mgL-1, Pseudomonas aeruginosa exhibited the lowest removal efficiency of 35%, while Alcaligenes faecalis showed the highest removal efficiency of 60%. In the combined cultivation of Azolla and bacteria, for an initial TPH concentration of 1000 mgL-1, Pseudomonas aeruginosa and Azolla exhibited the lowest removal efficiency of 80%, while Alcaligenes faecalis and Azolla showed the highest removal efficiency of 100%. Combined cultivation Azolla and bacteria was effective to improve removal of petroleum hydrocarbon from water.

Citation:

Ramazan ali Dianati tilaki, Removal Of Petroleum Hydrocarbon From Water By Combined Cultivation Of Azolla And Bacteria. Journal of Environmental And Sciences 2025.