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Correspondence to Author: Yiping Hu, 

a Heilongjiang Academy of Agricultural Sciences, Harbin, China.

Abstract :
 Superinfection exclusion (SIE) is a mechanism that many viruses utilize to prevent other viruses, or viruses that are closely related to them, from entering or replicating in the cells they occupy. When a host plant infected with a weak strain of a virus or viroid develops immunity against a more severe strain that is closely similar to the first infectant, this phenomenon is known as SIE, also known as cross-protection. It is unclear how cross-protection works in its entirety. We conducted a comparative transcriptome analysis of potato (Solanum tuberosum L.) leaves in this investigation. We will henceforth refer to the strains PVYN− Wi-HLJ-BDH-2 and PVYNTN− NW-INM-W-369-12 as BDH and 369, respectively. Between the Control and JZ, 806 differentially expressed genes (DEGs) were found.(BDH preinfection and 369 treatment challenge). The response to external biological stimulation, signal transduction, kinase, immune, and redox pathways were all considerably enriched, according to a Gene Ontology (GO) analysis. We found a large number of metabolites that were expressed differently along these routes.connected to a viral illness. Furthermore, a small group of genes that are probably crucial for the development of cross-protection were also found in our data. In particular, we found significant differences in the expression of the subtilisin-like protease StSBT1.7 gene, elongation factor 1-alpha-like gene, and A1-II gamma-like gene; of these, StSBT1.7 was the most important in our transcriptome data. These genes have the ability to generate chemical defense in plants, promote the expression of defensive plant genes,and contribute to the generation of harmful microorganisms and trauma.

Introduction :
After rice, wheat, and corn, potatoes are the fourth most widely cultivated food crop worldwide. However, potato virus Y (PVY) significantly hinders potato growth and output. PVY is a common and economically harmful potato disease that belongs to the family Potyviridae and genus Potyvirus. There are various strain groupings within PVY, including as the traditional PVYO, PVYN, and PVYC strains. There are other strains and sub-strains of the virus, such as PVYO, PVYN, and PVYC (Jones, 1990; Zaitlin, 1976; Valkonen and Jari, 2015) and the recently emerged recombinant strains PVYN:O, PVYN− Wi, Eu-PVYNTN, and PVYNTN− NW (Karasev and Gray, 2013). On the other hand, in most, if not all, potato cultivars, they show wildly disparate levels of virulence, with PVYN− Wi typically producing mild symptoms and PVYNTN− NW-SYR-II producing severe symptoms (Bai et al., 2019; Chikh-Ali et al., 2010; Kamangar et al., 2014; Anfoka et al., 2014; Folimonova, 2013). PVYNTN− NW-SYR-II can cause potato tuber necrotic ringspot disease (PTNRD) in susceptible cultivars in addition to the foliar symptoms. This can have a disastrous effect on the yield and quality of potatoes (Mackenzie et al., 2019; McKinney, 1941; Nanayakkara et al., 2012).

Citation:

Yiping Hu.Analysis of the potato virus Y transcriptome reveals the synergistic pathways between two strains in Solanum tuberosum. The Journal of Virology 2024.