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• Case Report
• Open Access
• Chloe Boer

For many synthetic biologists, the ability to quickly and modularly assemble DNA components is essential. Golden Gate assembly can accomplish this, but it frequently necessitates the purchase and delivery of fresh primers for every component and assembly arrangement. For Golden Gate assembly, we present a minimal set of primers that can be used to amplify any DNA from the Registry of Standard Biological Parts.

• Editorial
• Open Access
• Daruen K. Grifin

We were all impacted by the global coronavirus outbreak, and cytogenetic conferences were no different. Both the 24th International Colloquium in Animal Cytogenetics and Genomics (ICACG) in Canterbury and the 23rd International Chromosome Conference (ICC) were scheduled for 2021 in Canberra.

• Case Report
• Open Access
• Susinna Neghsolo

Renal cysts and diabetes syndrome are linked to the very uncommon proximal 17q12 microdeletion, which includes the deletion of the HNF1B gene (RCAD). Numerous phenotypes, including as diabetes and renal cysts, are brought on by this chromosomal rearrangement and are consistent with Mullerian aplasia/dysgenesis, autism spectrum disorder and schizophrenia, learning disabilities, speech delay, transient newborn hypercalcemia, neonatal cholestasis, and maturity-onset diabetes of the young type 5 (MODY5).

• Research Article
• Open Access
• Megun A. Czelski

The renowned cell researcher Sally Hughes Schrader originally used the phrase “pre-metaphase stretch” in 1950 to refer to the elongation of prometaphase chromosomes seen in the early spermatocytes of praying mantids and phasmid insects.

• Research Article
• Open Access
• Sanra Elisa Bülau

There are numerous uses for karyotypic analysis in cytotaxonomy, evolution, and chromosome organization research. Additionally, they are necessary for genome assembly initiatives. Here, we use traditional staining with Giemsa and 18S rDNA probes to describe the karyotype of the endangered yellow cardinal, Gubernatrix cristata (Passeriformes, Thraupidae), for the first time.12 pairs of macrochromosomes and 27 pairs of microchromosomes make up this species’ 78 chromosomes.