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Correspondence to Author: Chloe Boer, Christa Habin, Deun Camenates. 

Alma College, 614 W. Superior St, Alma, MI 48801, USA.

Abstract:

For many synthetic biologists, the ability to quickly and modularly assemble DNA components is essential. Golden Gate assembly can accomplish this, but it frequently necessitates the purchase and delivery of fresh primers for every component and assembly arrangement. For Golden Gate assembly, we present a minimal set of primers that can be used to amplify any DNA from the Registry of Standard Biological Parts. Although these primers pair improperly, they add type IIS restriction enzyme sites in a manner that reduces assembly scars and bind to regions that are common to the backbone plasmid for these components. This method speeds up and lowers the cost of redesigning assembly procedures, which can allow more scientists and students have access to synthetic biology.

Keywords:Golden Gate; BioBrick; registry; PCR; iGEM; universal; primers.

Citation:

Dr.Chloe Boer, Gem-Gate: An Affordable, Adaptable Method For Biobrick Construction. Journal of DNA Research 2025.