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Correspondence to Author: Anam Niazi. 

nstitute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan.

DOI: 10.52338/joid.2026.5601

Abstract:

Introduction: Since bacteria which can penetrate and reproduce within phagocytes are more likely to cause prolonged and serious infections, this emphasize the use of those antibiotics which concentrate inside cells and kill those bacteria. C/E ratio is a parameter to determine the drug concentration inside and outside the cell. In present study we determined the C/E Ratios of doxycycline, enoxacin and roxithromycin in human polymorphonuclear neutrophils.
Method: Polymorphonuclear neutrophils from the whole blood was separated by the protocol given by Polymorphprep reagent obtained from Progen Company with few modifications. Viability of cells was tested by trypan blue. Later, these neutrophils were allowed to incubate with drugs at concentration prepared by given C max and T max in literature. After that they were micro-centrifuge, freezed at -80 ͦC and hence been separated into cellular and extracellular concentrations which were quantitatively assayed by RP-High Performance Liquid Chromatography technique.
Results: The result shows that doxycycline, enoxacin and roxithromycin have an outstanding ability to concentrate inside the human polymorphonuclear neutrophils. Hence these drugs are proven to be more effective against intracellular pathogens. However, they are dependent upon the conditions and factors provided to them. These results can prove to be an important milestone for further research and are of great importance. It provides us a fair idea towards treatment approach and allows the comparison with other drugs of same group.
Conclusion: This study demonstrates that enoxacin and doxycycline achieve substantially higher intracellular accumulation in human polymorphonuclear neutrophils than roxithromycin, supporting their greater potential efficacy against intracellular pathogens.

Keywords: Polymorphonuclear neutrophils; Intracellular accumulation; Doxycycline; Enoxacin; Roxithromycin.

Citation:

Dr. Anam Niazi, Cellular To Extra-Cellular Concentrations Of Doxycycline, Enoxacin And Roxithromycin In Human Polymorphonuclear Neutrophils. Journal of Infectious Diseases 2026.