Single-Cell Sequencing Reveals a Regulatory Role of WT1 in Mast Cell Proliferation in Endometrial Polyps

Clinical Endocrinology; Metabolism Single

doi:10.52338/jocem.2025.4404

Abstract

Endometrial polyps are the predominant structural anomalies of the endometrial mucosa observed in unexplained infertility cases, potentially compromising endometrial receptivity and suggesting shared etiological characteristics. However, their comprehensive cell atlas and immune landscape remain inadequately defined. In this study, we employed single-cell RNA sequencing and bulk RNA- seq to systematically analyze ectopic endometrial polyps (EPs) alongside adjacent eutopic endometrial tissues (EUs). This enabled us to delineate alterations in cell composition and transcriptional dynamics across diverse cell types associated with endometrial polyps. Notably, we observed an increase and activation of mast cells, with significant transcriptional profile variations. Through transcription regulatory network analysis, WT1 was identified as a pivotal transcriptional regulator mediating mast cell proliferation in EPs, concomitant with the dysregulation of WT1 target genes involved in cell growth. These findings provide novel insights into the cellular heterogeneity and molecular mechanisms of endometrial polyps at single-cell resolution, presenting potential therapeutic targets for clinical intervention.

Keywords: Endometrial Polyp Single-Cell RNA Sequencing Mast Cells WT1

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Introduction

Endometrialpolyps(EPs)arelocalized,sessile,orpedunculated structural abnormality of the mucous membrane 1 . They are the most common structural abnormality of the mucous membrane of the endometrium detected in about 50% of cases of abnormal uterine bleeding and 35% of infertility2,3 . The risk factors for endometrial polyps’ development are tamoxifen usage4 , advanced age, hypertension, and obesity5,6 . Though malignant transformation of EPs is rare7,8 , the endometrial polyp provides an unfavorable environment for the endometrium receptivity and other etiopathogeneses. However,thepathogenesisofEPswasnotclear9 ,andtheexact cause of endometrial polyps is uncertain10 , One hypothesis suggests that the elevated levels of estrogen receptors (ERs) in the glandular cells of polyps are significantly higher than those found in normal endometrial tissue11 .

Studies also supposed it as endometrial localized chronic inflammation and mast cells might be essential to initiate and control the inflammation through cytokines and growth factors secretion. However, the cell landscape of endometrial polyps is still uncertain. Evaluation of the endometrial cells and epithelial evolutionary trajectory in patients with EPs is an important line of investigation in the pathophysiology of the disease. Singlecell RNA sequencing (scRNA-seq) has become an essential technique for analyzing the complexity and diversity within tissues, offering insights into the cellular and molecular landscapes of complex tissues. By profiling both ectopic endometrial polyps and adjacent eutopic endometrium, this Abstract Endometrial polyps are the predominant structural anomalies of the endometrial mucosa observed in unexplained infertility cases, potentially compromising endometrial receptivity and suggesting shared etiological characteristics.

However, their comprehensive cell atlas and immune landscape remain inadequately defined. In this study, we employed single-cell RNA sequencing and bulk RNAseq to systematically analyze ectopic endometrial polyps (EPs) alongside adjacent eutopic endometrial tissues (EUs). This enabled us to delineate alterations in cell composition and transcriptional dynamics across diverse cell types associated with endometrial polyps. Notably, we observed an increase and activation of mast cells, with significant transcriptional profile variations. Through transcription regulatory network analysis, WT1 was identified as a pivotal transcriptional regulator mediating mast cell proliferation in EPs, concomitant with the dysregulation of WT1 target genes involved in cell growth. These findings provide novel insights into the cellular heterogeneity and molecular mechanisms of endometrial polyps at single-cell resolution, presenting potential therapeutic targets for clinical intervention.

Keywords : Endometrial polyp; Single-cell RNA sequencing; Mast cells; WT1. approach allows for a detailed comparison of the cellular dynamics and transcriptional alterations specific to EPs. Mast cells serve a pivotal role in the pathobiology of type 2 (T2) allergic inflammatory airway diseases, such as asthma and chronic rhinosinusitis with nasal polyps12 . These factors contribute to the advancement and intensity of type 2 inflammatory disorders through various mechanisms, such as the synthesis of eicosanoids, the production of cytokines, and the secretion of pre-existing mediators like proteases and histamine13 . The expansion of Mast cells is a notable characteristic of both nasal and colonic polyps. Research suggests that the growth of these polyps is partially driven by the local proliferation of mast cells within the nasal polyp tissue14 .

Furthermore, a high density of activated Mast cells has been reported in endometrial polyps15 . Therefore, identifying the factors that stimulate mast cells proliferation holds significant pathobiologic implications for endometrial polyps. Given these insights, elucidating the transcriptional regulation and cellular interactions within endometrial polyps (EPs) is pivotal for understanding their pathophysiology. Here, we identify transcriptional regulators, notably WT1, as central to mast cell proliferation. This study seeks to delineate the role of WT1 in this proliferation process and its broader implications for EPs formation and progression. By dissecting transcriptional regulatory programs and cellular dynamics, we aim to uncover novel therapeutic targets for addressing EPs-associated symptoms and complications.

Methods

Patients and samples In this study, all samples were obtained from the international maternity and child health hospital of Medical school, Shanghai Jiaotong University, Shanghai, China. The detailed patients and sample information can be found in Table S1.Informed consent from patients were obtained before this work. All procedures involved were approved by the Institutional Review Board of international maternity and child health hospital. Preparation of single cell suspensions All endometrial tissue samples were processed independently using an identical protocol. In brief, the tissues were minced into small fragments, each measuring less than 1 mm in diameter. These fragments underwent enzymatic digestion with collagenase I (1 mg/ml; Thermo Fisher) and deoxyribonuclease I (0.2 mg/ml; Thermo Fisher) for 50 minutes on a rotator at 37 °C.

The resulting digested mixture was filtered through a 70-μm cell strainer to isolate dissociated cells. Following this, the supernatant was discarded after centrifugation at 300g for 10 minutes, and the pellet was resuspended in 1 mL of red blood cell lysis buffer (Thermo Fisher) and incubated at 4 °C for 10 minutes to eliminate intact red blood cells. To further purify the single-cell suspension, a Dead Cell Removal Kit (Miltenyi) was employed to eliminate dead cells. The resultant pellet was then resuspended in 5 mL of calcium- and magnesium-free PBS containing 0.04% weight/volume BSA. Finally, 10 µl of this suspension was counted using a hemocytometer under an inverted microscope, with Trypan blue utilized to distinguish live cells.

After confirming the quality of the single-cell suspensions, three samples from each group were selected for 10x Genomic sequencing, given the high costs associated with single-cell RNA sequencing. Library construction for single cell gene expression Six samples were processed using the 10x Genomics Chromium platform , as detailed in Table S1. The freshly preparedsingle-cellsuspensionwasdilutedtoaconcentration of 700-1200 cells per μl, following the guidelines provided in the 10x Genomics Chromium Next GEM Single Cell 3’ Reagent Kits v3.1 (No. 1000268) for computational analysis and library construction. The resulting library was sequenced on the Illumina Nova 6000 PE150 platform. Furthermore, eight samples were examined with the BD Rhapsody™ platform, as outlined in Table S1.

In accordance with the manufacturer’s guidelines, the single-cell suspension was modified to the correct volume for processing and capture using the BD Rhapsody™ Enhanced Cartridge Reagent Kit (Cat. No. 664887) along with the BD Rhapsody™ Cartridge Kit (Cat. No. 633733). Reverse transcription was carried out utilizing the BD Rhapsody™ cDNA kit (Cat. No. 633773), and the BD Rhapsody™ WTA Amplification Kit (Cat. No. 633801) was employed for constructing the DNA library. High-throughput sequencing was performed in PE-150 mode. Processing Raw Data from scRNA-seq The FASTQ files from 10xGenomics Chromium platform were initially analyzed using the 10xGenomics Chromium pipeline, employing the default parameters from the Cell Ranger single-cell software suite (v6.1.2) (10xGenomics) (accessible at https://www.10xgenomics.com/support/software/cellranger/latest).

Following this, the FASTQ files from the BD Rhapsody™ platform were processed via the BD Rhapsody WTA analysis pipeline, utilizing the GRCh38 human genome as a reference (Seven Bridges, Charlestown, Mass). The resulting data were subsequently evaluated using SeqGeq 1.8.0 (BD Biosciences). Single-cell gene expression quantification and determination of cell types The single-cell RNA-seq data were analyzed using the Seurat R package (version 4.0.4). To exclude doublets, the R package DoubletFinder was employed. After conducting quality control, a total of 103,539 cells were retained for subsequent bioinformatics analyses. To mitigate batch effects between samples and experiments, integration was performed using the fastMNN method. Dimensionality reduction was achieved with the “RunUMAP” function, allowing visualization through Uniform Manifold Approximation and Projection (UMAP).

For subgroup clustering, cells of various types were separately extracted and clustered based on their first 22 clusters, using a resolution of 0.5. The Wilcoxon rank-sum test, executed with the “FindAllMarkers” function, identified markers for each cluster. Only genes with |avg_log2FC| > 0.25 and p_val_adj < 0.05 were considered significant marker genes. The marker genes for each cluster are detailed in Supplementary Table S2. Bulk RNA sequencing and data analysis To identify the genes responsible for endometrial polyps, we compare the expression profiles between 5 endometrial polyps and 5 eutopic endometrium. Adapter trimming and quality control were automated using Trim Galore (version 0.6.7). For genomic alignment, Hisat2 (version 2.1.0) was utilized to map sequences to the human reference genome (GRCh38).

The read counts for each gene were calculated using featureCounts (version 1.6.3). Differentially expressed genes between the samples were determined using DEGseq2, applying thresholds of |Log2-fold change| > 1 and a p-value < 0.05. Cellular Communication Networks in Single-Cell Sequencing Data To explore enhanced cell-cell communication between mast cellsandothercells,weutilizedCellPhoneDB,acomprehensive tool designed to identify and visualize cell-type interactions based on signaling characteristics. we employed the METHOD 3: Retrieval of Differentially Expressed Interactions available in CellPhoneDB version 316 , which leverages differentially expressed genes to extract and highlight enhanced cell communication. WT1 knockdown with siRNAs To investigate the role of WT1 in mast cell proliferation, we performed siRNA-mediated knockdown of WT1 in the HMC-1 mast cell line.

The experiments were conducted in triplicate, with three samples undergoing WT1 knockdown and three control samples treated with a non-targeting siRNA. RNA sequencing was carried out to assess changes in gene expression 24 hours post-transfection. Following transfection, cell counts were measured at 0, 24, and 48 hours using CounterSTAR (ALIT Biotech, China). Identiﬁcation of DEGs between endometrial polyps and eutopic endometrial tissue To identify differentially expressed genes (DEGs), we utilized the “FindMarkers” function in Seurat. The log fold change (Log2FC) and the adjusted p-values for each DEG were evaluated through ROC analysis. Only genes that met the criteria of |avg_logFC| > 0.25 and FDR (false discovery rate) indicated by p_val_adj < 0.05 were classified as DEGs.

Transcriptional Regulatory Network Analysis The transcriptional regulatory network was examined using the GENIE3 (version 1.10.0) and RcisTarget (version 1.8.0) R packages as part of the SCENIC workflow (version 1.1.2.1), applying the default settings (28). Reference transcription factors (TFs) corresponding to hg19 were obtained via RcisTarget. The gene expression data from 31,503 cells were normalized using Seurat and served as input.Initially, co-expression modules were identified by analyzing the relationships between transcription factors and potential target genes based on the gene expression data through GENIE3. Following this, cis-regulatory motif enrichment analysis was conducted on all potential target genes within each co-expression module using RcisTarget. Only those target genes that were enriched with the specific motifs of their corresponding TFs were designated as direct target genes.

Each transcription factor, along with its direct target genes, was categorized as a regulon. Finally, the gene regulatory interactions between fibroblast and epithelial cells were inferred using the Seurat FindAllMarkers function. Pathway Enrichment Analysis and Visualization It is was performed following a previous study17 , For pathway enrichment analysis and visualization of differentially expressed genes (DEGs), we followed a comprehensive protocoloutlinedbyReimandetal18.andutilizedMetascape19 . The initial step involved using g:Profiler to conduct pathway enrichment analysis on the DEG list. Subsequently, the results were visualized through EnrichmentMap (version 3.5.0) within Cytoscape (version 3.10.1). To facilitate the navigation and interpretation of the pathway enrichment outcomes, we employed AutoAnnotate (version 1.3.2) in Cytoscape. Metascape serves as an online platform that offers extensive resources for the annotation and analysis of gene lists.

Immunohistochemistry Fresh human specimens were fixed in a 4% paraformaldehyde solution for 24 hours, subsequently embedded in paraffin, and sectioned into 5 μm slices. These sections underwent deparaffinization in xylene and rehydration in ethanol. Antigen retrieval was conducted using Tris-EDTA (pH 9.0) (Biosharp, Anhui, China) in a microwave. For the subsequent procedures, a staining kit from Absin (Shanghai, China) was utilized.In brief, the slides were treated with 3% H₂O2 to inhibit endogenous peroxidase activity, followed by blocking with 5% BSA. The sections were then incubated overnight at 4 °C with primary antibodies targeting CD63 (1:200) and TPSB2 (1:200). This was followed by incubation with a secondary antibody. Staining was carried out using DAB and hematoxylin.

Observations and imaging of the slides were performed with a microscope (Leica, Germany). Immunoﬂuorescence Paraffin sections with a thickness of 7 μm were prepared. Following dewaxing and rehydration, heat-mediated antigen retrieval was conducted using a citrate-based solution (pH 6.0). The sections were subsequently permeabilized in PBS supplemented with 0.4% Triton X-100 for 30 minutes at room temperature. After washing with PBS, the samples were then blocked with 5% bovine serum albumin (BSA) at room temperature for 1 hour. Following this, they were incubated overnight at 4 with primary antibodies against trytase (1:100, Abcam, ab8978, Cambridge, UK),. After washing with PBS, the samples were treated with 555-conjugated secondary antibodies (Invitrogen, California, A-31572, USA) for 1 hour in the dark at room temperature, then stained with 4,6-diamidino-2-phenylindole (DAPI).

Immunofluorescence imaging was carried out using a confocal microscope (Leica, Germany). Statistics Cell distribution between the two groups was analyzed using unpaired two-tailed Wilcoxon rank-sum tests. For comparing gene expression or gene signatures between the two cell groups, unpaired two-tailed Student’s t-tests were employed. All statistical analyses and visualizations were conducted using R. The specific statistical tests applied in the figures are detailed in the figure legends, with statistical significance defined as p < 0.05.

Results

scRNA-seq profiling of endometrial polyps and eutopic endometrium tissues We performed single-cell RNA sequencing to construct a global endometrial polyps (EPs) atlas, which profiled both ectopic endometrial and adjacent eutopic endometrium tissues (EU) from seven patients with endometrial polyps (Fig.1A). In sections stained with hematoxylin and eosin (H&E), we noted a twofold increase in the cross-sectional area of the glandular epithelium in the MP group compared to the EU group (Fig 1B), After rigorous quality control, a total of 103,539 cells were identified, including 52974 cells from eutopic endometrium and 50565 cells from the polyps, respectively. Average 2840 genes and 8914 unique molecular identifiers (UMIs) were obtained per cell, indicating sufficient coverage and transcripts representative(Fig.

S1). Next, we performed unsupervised clustering analysis and genomic signature identification, 8 clusters were defined in the endometrium (Fig.1C and D), including epithelial cells (9.3 %), fibroblasts (67.1%), smooth muscle cells (SMCs) (2.5 %), endothelial cells (2.1 %), macrophages (3.8 %), NK cells (6.8 %), mast cells (1.1%), and CD8 T cells (7.2%). The number for each cell type in endometrial samples is shown in Table1. The correlation between these cell types confirms their identification (Fig. S2). Table 1 Patients` Information Sample Patient ID Age at Surgery (Years) History of gestation Phase of Cycle The cycle days Abnormal bleeding or not prior to Surgery (1=yes; 0=no) Treatment received prior to Surgery (1=Surgery treatment; 2=drug treatment; 0=untreated) Number of polyps Polyps location in uterus The diameter of the largest polyps(cm) Experiment No.cells analyzed EU1 1 47 1-1-0-0 proliferative 10/28 0 0 1 Right anterior 1.5 single cell RNAseq (BD Rhapsody) 5370 EP1 2519 EU2 2 41 5-2-3-0 proliferative 11/28 0 0 1 anterior 0.6 single cell RNAseq (BD Rhapsody) 4364 EP2 8526 EU3 3 29 0-0-0-0 proliferative 13/28 1 0 1 Right Lateral 1.2 single cell RNAseq (BD Rhapsody) 1091 EP3 1239 EU4 4 37 3-2-1-0 proliferative 11/30 0 0 1 anterior lateral 0.5 single cell RNAseq (BD Rhapsody) 6835 EP4 4592 EU5 5 42 1-1-0-0 proliferative 8/21 0 0 1 anterior 1.5 single cell RNAseq (10× Genomics) 12046 EP5 11254 EU6 6 27 0-0-0-0 proliferative 10/28- 1 0 1 left horn 0.5 single cell RNAseq (10× Genomics) 13068 EP6 11407 EU7 7 28 0-0-0-0 proliferative 13/30 0 0 1 posterior fundus 1 single cell RNAseq (10× Genomics) 10200 EP7 11028 EU8 8 37 1-0-1-0 proliferative 11/30 0 1 2 anterior, fundus 1.5 Bulk RNAseq EP8 EU9 9 35 1-0-0-1 proliferative 13/28 1 0 1 Right anterior 2.5 Bulk RNAseq EP9 EU10 10 34 0-0-0-0 proliferative 9/28 0 0 1 anterior 0.5 Bulk RNAseq EP10 EU11 11 28 1-0-1-0 proliferative 10/28- 0 0 1 anterior 0.5 Bulk RNAseq EP11 EU12 12 29 0-0-0-0 proliferative 11/30 1 0 1 fundus 1 Bulk RNAseq EP12 Next, we examined the changes in cell composition associated with endometrial polyps and discovered a reduction in the proportion of fibroblasts within these polyps, while the proportions of epithelial cells (p=0.049), mast cells (p=0.002), and macrophages (p=0.017) increased (Fig.

1E). To validate these changes in cell composition within the polyps, we performed immunohistochemistry staining using cell-specific markers. Indeed, we observed that the numbers of CD68-positive macrophages and TPSB2-positive mast cells in the polyps were approximately twice as high as those in the eutopic endometrium (Fig. 1F and G), consistent with previous reports , . Moreover, the levels of Ki67 in the glandular epithelium of the polyps were elevated, corroborating the increase in epithelial cells we had observed (Fig. 1H). Collectively, these observations suggest glandular proliferation and immune cell infiltration, including macrophages and mast cells, in the polyps, likely contributing to the proliferative and inflammatory alterations observed. Figure 1. Identification of Cell Types through scRNA-Seq Analysis of Endometrial Polyps.

(A) Schema: both ectopic endometrial polyps (EP) and adjacent eutopic endometrium tissues (EU) from seven patients with endometrial polyps were profiled with scRNA-seq. (B) Hematoxylin and eosin staining of EP and adjacent EU. Scale bars, 60 μm and 5 μm (zoomed-in images). Left, representative images. Right, quantitative analysis. (C) Uniform manifold approximation and projection (UMAP) plot showing the eight cell types of endometrial polyps. (D) Dot plot showing the expression of representative genes for each cell type. (E) Bar graph showing cell proportions (%) of various cell types in EU (solid red) and EP (light red) groups, with significant differences indicated by p-values. (F) Immunohistochemical staining for cell proliferation marker Ki67 in EU and EP.

Scale bars, 60 μm and 5 μm (zoomed-in images). Left, representative images. Right, the proportion of Ki67 positive cells was quantiffed. (G) Immunohistochemical staining for macrophage marker CD63 in EU and EP. Scale bars, 60 μm and 5 μm (zoomed-in images). Left, representative images. Right, the proportion of CD63 positive cells was quantified. (H) Immunohistochemical staining for mast cell marker TPSB2 in EU and EP. Scale bars, 60 μm and 5 μm (zoomed-in images). Left, representative images. Right, the proportion of TPSB2 positive cells was quantiffed. Aberrant gene expression in cell types from endometrial polyps’ samples To simultaneously define different gene expressions at the global and cellular levels, bulk RNA-seq was performed parallel for endometrial polyps and eutopic endometrium (Fig.2A and 2B).

94 upregulated and 16 downregulated genes were detected in endometrial polyps (log2FoldChange >1 & pvalue < 0.05 Supplemental Table2). Next, the GO analysis was performed. The results showed that regulation of inflammatory response, negative regulation of cell activation, and cellular lipid catabolic process associated signal pathway were activated in endometrial polyps. However, the genes involved in the proteoglycan metabolic process, neuron migration, and gas homeostasis were downregulated (Fig.2C). We assessed alterations in the expression of inflammation-associated genes within the cellular layers of endometrial polyps to explore particular anomalies in molecular expression across various cell types. Notably, CD163, which was found to be upregulated in the bulk RNA-seq analysis, showed increased expression specifically in macrophages, while its expression remained unchanged in other cell types.

Similarly, CST7, also upregulated in the bulk RNA-seq analysis, exhibited increased expression in mast cells, with no significant changes observed in other cells. Conversely, S100A9 and LPL, both upregulated in the bulk RNA-seq analysis, were found to be downregulated in macrophages and smooth muscle cells (SMCs) but remained unchanged in most other cell types (Fig.2D and 2E). These differences reflect the cellular heterogeneity in gene expression changes. Collectively, our findings highlight the complex and cell type-specific molecular landscape within endometrial polyps. Table 2. p_val avg_log2FC pct.1 pct.2 p_val_adj cluster gene change MT1X 3.95E-62 0.567729335 0.358 0.213 2.07E-57 CD8T MT1X up SCGB2A1 3.52E-201 0.542504173 0.354 0.059 1.84E-196 CD8T SCGB2A1 up WFDC2 8.42E-171 0.516935289 0.425 0.132 4.41E-166 CD8T WFDC2 up MT2A 2.74E-37 0.471645707 0.509 0.43 1.44E-32 CD8T MT2A up MT1E 3.64E-32 0.40759225 0.216 0.121 1.91E-27 CD8T MT1E up SCGB1D2 4.25E-81 0.281832434 0.173 0.033 2.22E-76 CD8T SCGB1D2 up RBP1 1.60E-68 -0.251456109 0.068 0.203 8.39E-64 CD8T RBP1 down DIO2 9.59E-63 -0.251718214 0.07 0.198 5.02E-58 CD8T DIO2 down COL3A1 4.60E-47 -0.259477593 0.397 0.56 2.41E-42 CD8T COL3A1 down ECM1 1.07E-90 -0.268129557 0.042 0.187 5.58E-86 CD8T ECM1 down LGALS1 3.17E-85 -0.291988872 0.622 0.786 1.66E-80 CD8T LGALS1 down MGP 6.24E-78 -0.303603062 0.095 0.254 3.27E-73 CD8T MGP down SFRP4 6.15E-76 -0.311830861 0.485 0.653 3.22E-71 CD8T SFRP4 down GNLY 6.23E-48 -0.327327307 0.179 0.335 3.26E-43 CD8T GNLY down SCGB1D21 3.92E-37 1.004224648 0.829 0.42 2.05E-32 ciliated_EpC SCGB1D2 up SCGB1D4 1.51E-28 0.876886452 0.777 0.401 7.92E-24 ciliated_EpC SCGB1D4 up TFF3 6.75E-27 0.850530063 0.739 0.408 3.54E-22 ciliated_EpC TFF3 up CHGA 2.89E-14 0.642833135 0.535 0.236 1.51E-09 ciliated_EpC CHGA up SCGB2A11 1.43E-37 0.508104213 0.988 0.885 7.51E-33 ciliated_EpC SCGB2A1 up WFDC21 3.55E-25 0.496883184 0.978 0.79 1.86E-20 ciliated_EpC WFDC2 up PAEP 1.59E-13 0.464580968 0.409 0.089 8.33E-09 ciliated_EpC PAEP up SOD3 1.87E-16 0.447220389 0.616 0.261 9.77E-12 ciliated_EpC SOD3 up SPP1 1.15E-14 0.44264295 0.355 0.045 6.01E-10 ciliated_EpC SPP1 up CFAP70 1.87E-15 0.424399087 0.732 0.465 9.80E-11 ciliated_EpC CFAP70 up LINC01480 1.20E-13 0.411553809 0.88 0.675 6.29E-09 ciliated_EpC LINC01480 up FXYD3 2.30E-10 0.403404512 0.559 0.287 1.21E-05 ciliated_EpC FXYD3 up EPHX1 3.15E-15 0.40190866 0.852 0.605 1.65E-10 ciliated_EpC EPHX1 up CLU 1.46E-13 0.39861419 0.8 0.605 7.64E-09 ciliated_EpC CLU up PIGR 3.33E-13 0.395426635 0.651 0.363 1.74E-08 ciliated_EpC PIGR up EPPIN 2.24E-14 0.387416063 0.62 0.28 1.17E-09 ciliated_EpC EPPIN up CD59 1.25E-14 0.386671247 0.764 0.478 6.52E-10 ciliated_EpC CD59 up BX890604.2 9.87E-12 0.369878311 0.601 0.376 5.17E-07 ciliated_EpC BX890604.2 up METRN 1.64E-14 0.3693577 0.863 0.713 8.56E-10 ciliated_EpC METRN up GALNT4 1.59E-13 0.367983813 0.438 0.146 8.32E-09 ciliated_EpC GALNT4 up GSTA3 3.83E-15 0.366017046 0.715 0.439 2.00E-10 ciliated_EpC GSTA3 up BMPR1B 6.37E-15 0.365954987 0.622 0.293 3.33E-10 ciliated_EpC BMPR1B up SYNE1 1.88E-12 0.363573242 0.728 0.465 9.84E-08 ciliated_EpC SYNE1 up MS4A8 4.32E-12 0.359314708 0.899 0.707 2.26E-07 ciliated_EpC MS4A8 up NDUFC2 1.54E-11 0.354029994 0.736 0.529 8.06E-07 ciliated_EpC NDUFC2 up POU2AF1 4.06E-14 0.353123814 0.491 0.185 2.13E-09 ciliated_EpC POU2AF1 up SRGAP3 1.70E-12 0.346248474 0.676 0.382 8.89E-08 ciliated_EpC SRGAP3 up WFDC6 1.40E-15 0.345344851 0.383 0.07 7.34E-11 ciliated_EpC WFDC6 up NT5E 2.22E-11 0.343168726 0.517 0.255 1.16E-06 ciliated_EpC NT5E up TMBIM4 1.89E-11 0.336422324 0.627 0.369 9.91E-07 ciliated_EpC TMBIM4 up CSRP2 7.02E-13 0.330425353 0.754 0.503 3.68E-08 ciliated_EpC CSRP2 up IFI27 1.17E-14 0.32942119 0.947 0.854 6.15E-10 ciliated_EpC IFI27 up PLPP2 6.79E-10 0.329262364 0.643 0.433 3.56E-05 ciliated_EpC PLPP2 up HOMER2 3.89E-11 0.328636327 0.781 0.497 2.04E-06 ciliated_EpC HOMER2 up ANKUB1 6.28E-11 0.325484649 0.677 0.452 3.29E-06 ciliated_EpC ANKUB1 up KCNN3 2.16E-12 0.323300188 0.616 0.338 1.13E-07 ciliated_EpC KCNN3 up ASRGL1 6.81E-13 0.314241989 0.924 0.758 3.57E-08 ciliated_EpC ASRGL1 up CD9 7.94E-11 0.3136687 0.771 0.529 4.16E-06 ciliated_EpC CD9 up NFIA 1.00E-11 0.313181006 0.742 0.471 5.24E-07 ciliated_EpC NFIA up C4orf48 2.63E-07 0.31153119 0.678 0.478 0.013787146 ciliated_EpC C4orf48 up GADD45G 7.97E-08 0.311328377 0.526 0.306 0.004172822 ciliated_EpC GADD45G up CP 1.68E-07 0.309994233 0.463 0.248 0.00878835 ciliated_EpC CP up AL365259.1 1.02E-11 0.309780351 0.496 0.236 5.34E-07 ciliated_EpC AL365259.1 up FABP6 4.15E-10 0.308634013 0.754 0.548 2.17E-05 ciliated_EpC FABP6 up PGR 7.59E-12 0.307362385 0.874 0.701 3.97E-07 ciliated_EpC PGR up HLA-DRA 5.66E-09 0.30592408 0.535 0.312 0.000296387 ciliated_EpC HLA-DRA up HLA-C 1.11E-08 0.305480166 0.714 0.541 0.000578906 ciliated_EpC HLA-C up GTF2I 1.43E-08 0.304650343 0.681 0.497 0.000749873 ciliated_EpC GTF2I up PAX8 9.81E-11 0.304380068 0.773 0.561 5.14E-06 ciliated_EpC PAX8 up CATSPERD 4.42E-10 0.302855809 0.604 0.369 2.31E-05 ciliated_EpC CATSPERD up ATOX1 1.46E-10 0.302577301 0.632 0.369 7.67E-06 ciliated_EpC ATOX1 up ENPP2 1.23E-10 0.301208676 0.506 0.242 6.43E-06 ciliated_EpC ENPP2 up FUCA1 2.21E-10 0.297786923 0.581 0.363 1.16E-05 ciliated_EpC FUCA1 up HYDIN 1.60E-09 0.295845261 0.771 0.554 8.37E-05 ciliated_EpC HYDIN up MICOS13 9.64E-10 0.29323782 0.733 0.51 5.05E-05 ciliated_EpC MICOS13 up UGT2B7 2.46E-11 0.292405926 0.553 0.261 1.29E-06 ciliated_EpC UGT2B7 up LINC00240 1.78E-10 0.291099255 0.614 0.376 9.31E-06 ciliated_EpC LINC00240 up SSPN 1.61E-10 0.289860913 0.613 0.357 8.42E-06 ciliated_EpC SSPN up BHLHE41 3.17E-10 0.289554162 0.716 0.459 1.66E-05 ciliated_EpC BHLHE41 up SCPEP1 4.20E-11 0.289424065 0.597 0.344 2.20E-06 ciliated_EpC SCPEP1 up SLC23A1 1.67E-08 0.288275825 0.693 0.497 0.00087406 ciliated_EpC SLC23A1 up C16orf89 7.89E-13 0.287977412 0.376 0.089 4.13E-08 ciliated_EpC C16orf89 up RNASEK 8.28E-06 0.286794411 0.63 0.42 0.43329628 ciliated_EpC RNASEK up KIAA1324 7.65E-10 0.285814107 0.641 0.369 4.00E-05 ciliated_EpC KIAA1324 up SYNE2 2.82E-09 0.283737064 0.773 0.618 0.000147506 ciliated_EpC SYNE2 up AC084866.1 6.16E-10 0.283123117 0.725 0.503 3.22E-05 ciliated_EpC AC084866.1 up ATP6V0C 2.60E-08 0.282169742 0.547 0.293 0.001362647 ciliated_EpC ATP6V0C up CATIP 4.41E-10 0.281889054 0.507 0.248 2.31E-05 ciliated_EpC CATIP up FBLN1 4.23E-07 0.281162874 0.728 0.503 0.022128103 ciliated_EpC FBLN1 up CFAP47 1.15E-09 0.28115668 0.656 0.42 6.02E-05 ciliated_EpC CFAP47 up PKHD1L1 6.77E-08 0.280380707 0.459 0.242 0.003544584 ciliated_EpC PKHD1L1 up MT-ATP6 1.51E-15 0.280233863 0.98 0.866 7.92E-11 ciliated_EpC MT-ATP6 up DNAH11 1.33E-08 0.278965344 0.729 0.535 0.000694931 ciliated_EpC DNAH11 up AKAP9 8.74E-09 0.277885063 0.877 0.662 0.000457778 ciliated_EpC AKAP9 up ALG1L 3.51E-10 0.274320309 0.528 0.261 1.84E-05 ciliated_EpC ALG1L up CCDC88A 1.39E-08 0.273844692 0.662 0.401 0.000725692 ciliated_EpC CCDC88A up SMIM19 1.66E-10 0.272050895 0.756 0.541 8.71E-06 ciliated_EpC SMIM19 up C11orf54 6.32E-10 0.27100599 0.479 0.248 3.31E-05 ciliated_EpC C11orf54 up C2orf88 1.10E-07 0.2694555 0.774 0.503 0.005766478 ciliated_EpC C2orf88 up MT-ND3 8.10E-08 0.268640092 0.957 0.777 0.004242497 ciliated_EpC MT-ND3 up UACA 8.13E-09 0.267747805 0.766 0.599 0.000425535 ciliated_EpC UACA up TXNIP 2.46E-08 0.264443619 0.801 0.611 0.001289795 ciliated_EpC TXNIP up AL022068.1 6.40E-09 0.263874727 0.609 0.401 0.000334925 ciliated_EpC AL022068.1 up TMED6 2.66E-10 0.263271327 0.425 0.166 1.39E-05 ciliated_EpC TMED6 up IRX3 7.02E-09 0.263097091 0.7 0.439 0.000367338 ciliated_EpC IRX3 up DTHD1 2.44E-09 0.262677116 0.723 0.522 0.000127895 ciliated_EpC DTHD1 up ZNF486 4.50E-11 0.261081528 0.379 0.115 2.36E-06 ciliated_EpC ZNF486 up HLA-DRB1 1.97E-08 0.260618881 0.436 0.197 0.00103403 ciliated_EpC HLA-DRB1 up LSM7 7.97E-09 0.259347751 0.593 0.363 0.000417354 ciliated_EpC LSM7 up DNAJB13 2.24E-09 0.258670633 0.86 0.669 0.00011748 ciliated_EpC DNAJB13 up MT-CYB 1.27E-07 0.256746001 0.966 0.815 0.006626209 ciliated_EpC MT-CYB up NME2 1.65E-05 0.254958603 0.612 0.369 0.862828266 ciliated_EpC NME2 up RBBP7 2.28E-09 0.25444989 0.76 0.535 0.000119415 ciliated_EpC RBBP7 up NDUFA7 4.56E-07 0.254001605 0.582 0.344 0.023870433 ciliated_EpC NDUFA7 up ALDH1L1 1.70E-07 0.253719924 0.581 0.35 0.008877 ciliated_EpC ALDH1L1 up RESF1 1.42E-07 0.25321668 0.594 0.389 0.007419111 ciliated_EpC RESF1 up RARRES2 2.01E-08 -0.250115067 0.133 0.28 0.001054227 ciliated_EpC RARRES2 down MTATP6P1 0.004266311 -0.251699025 0.333 0.471 1 ciliated_EpC MTATP6P1 down TGFBI 2.88E-09 -0.255350676 0.092 0.236 0.00015105 ciliated_EpC TGFBI down SRGAP3-AS2 1.59E-09 -0.255925063 0.126 0.299 8.35E-05 ciliated_EpC SRGAP3-AS2 down SERPINH1 2.24E-07 -0.264627707 0.239 0.401 0.011742388 ciliated_EpC SERPINH1 down CNN3 6.45E-08 -0.265067355 0.214 0.382 0.00337836 ciliated_EpC CNN3 down IGF1 0.319987036 -0.265409812 0.36 0.344 1 ciliated_EpC IGF1 down GHITM 7.14E-08 -0.26881676 0.426 0.592 0.003738235 ciliated_EpC GHITM down ECM11 5.65E-08 -0.269619781 0.143 0.299 0.002956943 ciliated_EpC ECM1 down SPARC 0.07739704 -0.272868522 0.357 0.382 1 ciliated_EpC SPARC down DCN 1.21E-09 -0.272939081 0.11 0.268 6.35E-05 ciliated_EpC DCN down AC023300.2 1.72E-07 -0.282550052 0.603 0.682 0.00902502 ciliated_EpC AC023300.2 down COL6A1 2.67E-05 -0.283304034 0.255 0.376 1 ciliated_EpC COL6A1 down NREP 6.84E-06 -0.287553517 0.188 0.318 0.358311647 ciliated_EpC NREP down IGFBP2 9.85E-09 -0.291617428 0.205 0.376 0.000515481 ciliated_EpC IGFBP2 down VCAN 4.41E-05 -0.293311489 0.385 0.49 1 ciliated_EpC VCAN down MTND1P23 2.89E-06 -0.293355656 0.204 0.35 0.151284552 ciliated_EpC MTND1P23 down SMIM6 3.92E-08 -0.298711593 0.77 0.822 0.002053121 ciliated_EpC SMIM6 down NAPSB 1.42E-05 -0.305591431 0.095 0.204 0.74480578 ciliated_EpC NAPSB down ZNF295-AS1 2.35E-05 -0.323105309 0.573 0.605 1 ciliated_EpC ZNF295-AS1 down TAGLN2 6.66E-14 -0.338958633 0.811 0.854 3.49E-09 ciliated_EpC TAGLN2 down SFRP41 0.000250639 -0.339930509 0.714 0.707 1 ciliated_EpC SFRP4 down AC007906.2 2.93E-13 -0.344233792 0.82 0.873 1.54E-08 ciliated_EpC AC007906.2 down MT-RNR1 7.67E-06 -0.344288023 0.332 0.497 0.401646505 ciliated_EpC MT-RNR1 down LGALS11 0.000242185 -0.352766779 0.535 0.554 1 ciliated_EpC LGALS1 down MT-RNR2 0.000344453 -0.353107361 0.333 0.503 1 ciliated_EpC MT-RNR2 down COL1A1 1.31E-07 -0.372261919 0.749 0.803 0.006843584 ciliated_EpC COL1A1 down COL3A11 2.61E-07 -0.454589786 0.645 0.713 0.013649621 ciliated_EpC COL3A1 down MT-RNR21 6.64E-29 0.712782364 0.487 0.231 3.48E-24 Endothelia MT-RNR2 up MTATP6P11 1.22E-34 0.614257063 0.483 0.228 6.37E-30 Endothelia MTATP6P1 up MT-RNR11 9.88E-27 0.601966686 0.486 0.23 5.17E-22 Endothelia MT-RNR1 up CCL21 5.74E-21 0.598695576 0.176 0.03 3.01E-16 Endothelia CCL21 up CDKN1A 2.03E-43 0.571499244 0.619 0.357 1.07E-38 Endothelia CDKN1A up CLU1 1.62E-37 0.502550767 0.582 0.324 8.48E-33 Endothelia CLU up FOSB 3.13E-40 0.473071525 0.778 0.567 1.64E-35 Endothelia FOSB up ZFP36 6.87E-29 0.437483105 0.769 0.625 3.60E-24 Endothelia ZFP36 up NR4A1 3.54E-28 0.43477702 0.566 0.364 1.85E-23 Endothelia NR4A1 up SOCS3 2.64E-29 0.430143675 0.75 0.631 1.38E-24 Endothelia SOCS3 up BHLHE40 5.67E-23 0.413576294 0.628 0.488 2.97E-18 Endothelia BHLHE40 up KLF6 2.88E-32 0.409395492 0.792 0.627 1.51E-27 Endothelia KLF6 up EGR1 3.28E-25 0.392776331 0.726 0.601 1.72E-20 Endothelia EGR1 up PRSS23 1.09E-31 0.3902255 0.69 0.475 5.71E-27 Endothelia PRSS23 up KLF4 1.03E-25 0.383381228 0.617 0.409 5.38E-21 Endothelia KLF4 up GSN 9.45E-34 0.383259309 0.719 0.573 4.95E-29 Endothelia GSN up TFF31 7.08E-24 0.362721658 0.166 0.015 3.70E-19 Endothelia TFF3 up ATF3 7.44E-24 0.362453293 0.451 0.246 3.90E-19 Endothelia ATF3 up C2CD4B 4.57E-18 0.360461885 0.593 0.451 2.39E-13 Endothelia C2CD4B up FABP5 0.000194679 0.350649914 0.254 0.206 1 Endothelia FABP5 up HSPA1B 4.68E-16 0.349969768 0.639 0.522 2.45E-11 Endothelia HSPA1B up BTG2 6.95E-24 0.349785972 0.573 0.399 3.64E-19 Endothelia BTG2 up NRP2 5.88E-17 0.347953202 0.361 0.2 3.08E-12 Endothelia NRP2 up PDPN 2.81E-19 0.347533116 0.189 0.045 1.47E-14 Endothelia PDPN up RHOB 6.09E-23 0.346463552 0.731 0.567 3.19E-18 Endothelia RHOB up MT-ATP8 3.00E-18 0.337565352 0.702 0.596 1.57E-13 Endothelia MT-ATP8 up SLC2A3 3.51E-19 0.336456495 0.674 0.557 1.84E-14 Endothelia SLC2A3 up FABP4 1.23E-16 0.335509629 0.115 0.009 6.42E-12 Endothelia FABP4 up IGFBP5 6.12E-12 0.334759824 0.405 0.273 3.21E-07 Endothelia IGFBP5 up GDF15 1.26E-16 0.330820268 0.383 0.221 6.60E-12 Endothelia GDF15 up MAFF 7.92E-22 0.325277362 0.59 0.427 4.15E-17 Endothelia MAFF up CCL2 9.11E-13 0.322963937 0.442 0.31 4.77E-08 Endothelia CCL2 up IFI6 2.55E-20 0.322896547 0.733 0.627 1.34E-15 Endothelia IFI6 up LGALS3 3.47E-26 0.318605688 0.632 0.464 1.82E-21 Endothelia LGALS3 up IRF1 2.61E-18 0.313133481 0.721 0.636 1.37E-13 Endothelia IRF1 up SCGB2A12 1.07E-29 0.310676432 0.351 0.116 5.59E-25 Endothelia SCGB2A1 up TMEM70 2.77E-15 0.306044393 0.568 0.452 1.45E-10 Endothelia TMEM70 up DUSP5 1.64E-17 0.305266838 0.527 0.364 8.59E-13 Endothelia DUSP5 up TSPAN7 2.44E-19 0.302206557 0.501 0.345 1.28E-14 Endothelia TSPAN7 up TIMP3 4.03E-20 0.302155791 0.673 0.525 2.11E-15 Endothelia TIMP3 up ADIRF 5.92E-17 0.302149208 0.418 0.252 3.10E-12 Endothelia ADIRF up ICAM1 2.17E-13 0.294180582 0.53 0.397 1.14E-08 Endothelia ICAM1 up PROX1 2.54E-09 0.286447238 0.414 0.319 0.000132992 Endothelia PROX1 up SNHG5 1.38E-21 0.284714958 0.704 0.593 7.23E-17 Endothelia SNHG5 up SOCS2 2.83E-15 0.274425575 0.505 0.366 1.48E-10 Endothelia SOCS2 up JUND 1.02E-28 0.274309678 0.893 0.848 5.36E-24 Endothelia JUND up THBD 6.72E-14 0.269345569 0.57 0.43 3.52E-09 Endothelia THBD up GADD45B 5.56E-17 0.265845192 0.749 0.64 2.91E-12 Endothelia GADD45B up PGM5 8.04E-19 0.262333631 0.326 0.155 4.21E-14 Endothelia PGM5 up SLC25A25 5.75E-12 0.261774883 0.501 0.393 3.01E-07 Endothelia SLC25A25 up HSPA1A 4.70E-13 0.261362317 0.8 0.743 2.46E-08 Endothelia HSPA1A up BCL3 1.68E-11 0.260401412 0.521 0.412 8.79E-07 Endothelia BCL3 up MCL1 1.08E-19 0.259577074 0.782 0.701 5.66E-15 Endothelia MCL1 up PPP1R15A 1.67E-20 0.258468738 0.786 0.709 8.73E-16 Endothelia PPP1R15A up RPL26P19 4.15E-15 0.25450281 0.304 0.149 2.17E-10 Endothelia RPL26P19 up KLF2 6.14E-14 0.254229992 0.741 0.625 3.22E-09 Endothelia KLF2 up FOS 1.58E-17 0.252713848 0.871 0.8 8.27E-13 Endothelia FOS up WFDC22 2.94E-16 0.252603326 0.408 0.243 1.54E-11 Endothelia WFDC2 up SELENOP 3.72E-18 0.25246542 0.664 0.543 1.95E-13 Endothelia SELENOP up MSX1 5.17E-17 0.252113534 0.361 0.187 2.70E-12 Endothelia MSX1 up MATR3 6.63E-15 0.250775437 0.328 0.164 3.47E-10 Endothelia MATR3 up JUNB 3.63E-21 0.250192971 0.872 0.804 1.90E-16 Endothelia JUNB up MEIS2 8.77E-18 -0.252891831 0.521 0.691 4.59E-13 Endothelia MEIS2 down S100A13 9.87E-19 -0.253746508 0.511 0.691 5.17E-14 Endothelia S100A13 down ARL6IP4 1.22E-18 -0.256682546 0.386 0.6 6.36E-14 Endothelia ARL6IP4 down EDIL3 5.82E-26 -0.257122005 0.227 0.445 3.05E-21 Endothelia EDIL3 down CITED4 1.07E-17 -0.25894364 0.62 0.755 5.59E-13 Endothelia CITED4 down STARD4 1.06E-23 -0.262449887 0.257 0.475 5.54E-19 Endothelia STARD4 down RPS10 3.75E-14 -0.262567909 0.552 0.715 1.97E-09 Endothelia RPS10 down FN1 5.46E-21 -0.26368575 0.719 0.818 2.86E-16 Endothelia FN1 down HIST1H4C 8.20E-19 -0.265662583 0.389 0.564 4.30E-14 Endothelia HIST1H4C down VWA1 1.23E-22 -0.272872632 0.602 0.781 6.43E-18 Endothelia VWA1 down NEAT1 5.90E-18 -0.273520539 0.732 0.806 3.09E-13 Endothelia NEAT1 down COL4A1 1.38E-36 -0.273526035 0.851 0.913 7.21E-32 Endothelia COL4A1 down ARHGAP18 1.01E-20 -0.274127712 0.579 0.719 5.30E-16 Endothelia ARHGAP18 down GTF2I1 1.31E-22 -0.283690911 0.475 0.675 6.85E-18 Endothelia GTF2I down CASC15 1.31E-20 -0.286733097 0.336 0.512 6.86E-16 Endothelia CASC15 down MLEC 7.62E-28 -0.29709997 0.65 0.799 3.99E-23 Endothelia MLEC down SLC39A10 6.11E-24 -0.301243554 0.583 0.746 3.20E-19 Endothelia SLC39A10 down NME21 1.60E-18 -0.30240219 0.471 0.676 8.40E-14 Endothelia NME2 down NFAT5 1.91E-23 -0.305341257 0.558 0.713 1.00E-18 Endothelia NFAT5 down FAM167B 2.60E-27 -0.326613556 0.282 0.5 1.36E-22 Endothelia FAM167B down MIF 4.44E-21 -0.326791867 0.492 0.724 2.33E-16 Endothelia MIF down APCDD1 1.01E-35 -0.333608017 0.676 0.843 5.31E-31 Endothelia APCDD1 down EIF4A1 2.85E-25 -0.340953261 0.465 0.697 1.49E-20 Endothelia EIF4A1 down IGFBP21 1.14E-32 -0.341656465 0.584 0.77 5.96E-28 Endothelia IGFBP2 down EEF1G 3.06E-22 -0.342897308 0.468 0.701 1.60E-17 Endothelia EEF1G down CCDC85B 2.10E-34 -0.350542775 0.538 0.772 1.10E-29 Endothelia CCDC85B down RPS17 2.42E-27 -0.353560357 0.451 0.688 1.27E-22 Endothelia RPS17 down HAPLN1 3.17E-35 -0.370596557 0.123 0.348 1.66E-30 Endothelia HAPLN1 down RPL17 3.35E-22 -0.372417783 0.504 0.755 1.75E-17 Endothelia RPL17 down RPL36A 9.10E-29 -0.384275795 0.498 0.74 4.76E-24 Endothelia RPL36A down VCAN1 1.90E-22 -0.397996047 0.424 0.591 9.97E-18 Endothelia VCAN down DPEP1 1.11E-54 -0.472558932 0.193 0.494 5.83E-50 Endothelia DPEP1 down CCND1 0 0.72224076 0.526 0.113 0 Fibro CCND1 up HSPB6 0 0.609489377 0.753 0.443 0 Fibro HSPB6 up IGFBP6 0 0.588384908 0.509 0.249 0 Fibro IGFBP6 up HMGA1 0 0.574310998 0.359 0.071 0 Fibro HMGA1 up CDKN1A1 0 0.535796883 0.499 0.189 0 Fibro CDKN1A up PLAC9 0 0.527456845 0.48 0.229 0 Fibro PLAC9 up GDF151 0 0.515856767 0.355 0.085 0 Fibro GDF15 up TIMP31 0 0.477999935 0.717 0.4 0 Fibro TIMP3 up SOCS31 0 0.471885836 0.66 0.525 0 Fibro SOCS3 up S100A4 0 0.425199005 0.508 0.249 0 Fibro S100A4 up GPNMB 0 0.401776149 0.565 0.305 0 Fibro GPNMB up NKAIN4 0 0.398727113 0.306 0.015 0 Fibro NKAIN4 up CFD 0 0.391043815 0.299 0.078 0 Fibro CFD up ZMAT3 0 0.388106488 0.455 0.201 0 Fibro ZMAT3 up FXYD6 0 0.370193132 0.467 0.196 0 Fibro FXYD6 up CKB 0 0.365190431 0.876 0.762 0 Fibro CKB up APOE 0 0.349651052 0.794 0.693 0 Fibro APOE up PTN 0 0.343137589 0.671 0.513 0 Fibro PTN up SCGB2A13 0 0.342294552 0.369 0.075 0 Fibro SCGB2A1 up COL18A1 0 0.33394017 0.538 0.317 0 Fibro COL18A1 up HSPA1B1 0 0.333319151 0.611 0.487 0 Fibro HSPA1B up TGM2 0 0.331436126 0.729 0.56 0 Fibro TGM2 up CLU2 0 0.325715571 0.504 0.295 0 Fibro CLU up DNAJB1 0 0.324166235 0.785 0.676 0 Fibro DNAJB1 up EMP3 0 0.32330593 0.76 0.64 0 Fibro EMP3 up GSN1 0 0.316255528 0.826 0.704 0 Fibro GSN up FBLN2 0 0.315782486 0.518 0.261 0 Fibro FBLN2 up ZFP361 0 0.310341886 0.742 0.643 0 Fibro ZFP36 up NFIX 0 0.296543414 0.637 0.46 0 Fibro NFIX up LMNA 0 0.295878997 0.836 0.716 0 Fibro LMNA up PAPPA 0 0.294783107 0.45 0.244 0 Fibro PAPPA up PGF 0 0.293796842 0.249 0.05 0 Fibro PGF up AEBP1 0 0.285901997 0.612 0.464 0 Fibro AEBP1 up HSPA1A1 0 0.283955225 0.783 0.699 0 Fibro HSPA1A up DUSP1 0 0.281946785 0.779 0.688 0 Fibro DUSP1 up PLAG1 0 0.28183485 0.258 0.022 0 Fibro PLAG1 up WFDC23 0 0.280872098 0.393 0.183 0 Fibro WFDC2 up ATF31 0 0.277934728 0.537 0.399 0 Fibro ATF3 up FAM171A2 0 0.272030112 0.347 0.138 0 Fibro FAM171A2 up BTG21 0 0.26581098 0.672 0.548 0 Fibro BTG2 up C11orf96 0 0.260995148 0.715 0.565 0 Fibro C11orf96 up EFEMP1 0 0.260317056 0.356 0.215 0 Fibro EFEMP1 up MSX11 0 0.259588502 0.392 0.186 0 Fibro MSX1 up KLF21 0 0.257394477 0.39 0.23 0 Fibro KLF2 up DES 0 0.255971667 0.206 0.017 0 Fibro DES up METTL7A 0 0.253923511 0.461 0.273 0 Fibro METTL7A up KCTD12 0 0.253873022 0.455 0.28 0 Fibro KCTD12 up PPP1R1B 0 0.25251945 0.177 0.009 0 Fibro PPP1R1B up COL4A5 0 0.251430389 0.399 0.214 0 Fibro COL4A5 up SLC16A1 0 -0.253881461 0.266 0.481 0 Fibro SLC16A1 down WNT5A 0 -0.266862149 0.453 0.667 0 Fibro WNT5A down P2RY14 0 -0.266894672 0.363 0.566 0 Fibro P2RY14 down VWC2 0 -0.268171107 0.287 0.51 0 Fibro VWC2 down PRSS12 0 -0.274907831 0.153 0.396 0 Fibro PRSS12 down CD24 0 -0.280144167 0.689 0.854 0 Fibro CD24 down MMP11 0 -0.281070552 0.727 0.903 0 Fibro MMP11 down ANKRD29 0 -0.281934736 0.259 0.48 0 Fibro ANKRD29 down SLC5A3 0 -0.282979941 0.486 0.682 0 Fibro SLC5A3 down APCDD11 0 -0.283992676 0.569 0.774 0 Fibro APCDD1 down TNC 0 -0.286641049 0.274 0.472 0 Fibro TNC down S100A131 0 -0.287165276 0.62 0.747 0 Fibro S100A13 down SDK1 0 -0.289562228 0.434 0.661 0 Fibro SDK1 down LHFPL6 0 -0.307284843 0.276 0.514 0 Fibro LHFPL6 down OGN 0 -0.310055811 0.656 0.836 0 Fibro OGN down MGP1 0 -0.315710409 0.684 0.81 0 Fibro MGP down MARCKS 0 -0.317034423 0.766 0.907 0 Fibro MARCKS down LEF1 0 -0.318081475 0.252 0.519 0 Fibro LEF1 down TUNAR 0 -0.319898153 0.197 0.439 0 Fibro TUNAR down RBP7 0 -0.341655542 0.275 0.519 0 Fibro RBP7 down CFI 0 -0.345066959 0.233 0.5 0 Fibro CFI down NREP1 0 -0.355782307 0.738 0.926 0 Fibro NREP down VCAN2 0 -0.35939554 0.744 0.914 0 Fibro VCAN down ADAM12 0 -0.362118285 0.454 0.721 0 Fibro ADAM12 down RBP11 0 -0.37699359 0.684 0.86 0 Fibro RBP1 down DIO21 0 -0.3820085 0.729 0.911 0 Fibro DIO2 down PCSK5 0 -0.391757243 0.447 0.712 0 Fibro PCSK5 down GLIPR1 0 -0.401001424 0.425 0.654 0 Fibro GLIPR1 down IL32 0 -0.445618448 0.207 0.52 0 Fibro IL32 down TGFBI1 0 -0.459833685 0.553 0.814 0 Fibro TGFBI down ADAMTS9 0 -0.46849397 0.43 0.73 0 Fibro ADAMTS9 down F13A1 0 -0.573862967 0.181 0.575 0 Fibro F13A1 down ECM12 0 -0.622991688 0.654 0.938 0 Fibro ECM1 down SCGB2A14 4.07E-61 0.386437874 0.371 0.11 2.13E-56 Macrophages SCGB2A1 up WFDC24 2.95E-44 0.342779205 0.416 0.192 1.54E-39 Macrophages WFDC2 up HLA-DRB5 2.52E-15 0.317092636 0.345 0.23 1.32E-10 Macrophages HLA-DRB5 up KLF41 1.37E-38 0.29101541 0.842 0.729 7.20E-34 Macrophages KLF4 up SELENOP1 3.97E-11 0.266876374 0.501 0.416 2.08E-06 Macrophages SELENOP up LGALS12 9.64E-39 -0.264005014 0.762 0.894 5.05E-34 Macrophages LGALS1 down DIO22 5.04E-31 -0.267370301 0.195 0.372 2.64E-26 Macrophages DIO2 down ECM13 1.59E-42 -0.278004993 0.144 0.338 8.30E-38 Macrophages ECM1 down COL3A12 1.74E-21 -0.28207872 0.668 0.807 9.09E-17 Macrophages COL3A1 down MGP2 1.13E-40 -0.319907923 0.214 0.419 5.91E-36 Macrophages MGP down SFRP42 4.21E-43 -0.370587732 0.62 0.785 2.20E-38 Macrophages SFRP4 down RPL171 1.52E-13 0.900876443 0.674 0.193 7.97E-09 Mast_cells RPL17 up RPL36A1 2.06E-13 0.827926297 0.679 0.205 1.08E-08 Mast_cells RPL36A up RPS101 3.63E-14 0.817718054 0.681 0.261 1.90E-09 Mast_cells RPS10 up LTC4S 8.46E-14 0.744113675 0.672 0.239 4.43E-09 Mast_cells LTC4S up CTSG 1.60E-09 0.703938123 0.567 0.25 8.37E-05 Mast_cells CTSG up EEF1G1 4.18E-11 0.702077019 0.604 0.182 2.19E-06 Mast_cells EEF1G up HSPE1 8.76E-13 0.701099114 0.658 0.341 4.59E-08 Mast_cells HSPE1 up GABARAP 3.29E-12 0.69498763 0.592 0.182 1.72E-07 Mast_cells GABARAP up NME22 3.47E-12 0.691885646 0.596 0.182 1.82E-07 Mast_cells NME2 up ATP6V0C1 3.37E-12 0.682749403 0.527 0.136 1.77E-07 Mast_cells ATP6V0C up RNASEK1 8.03E-12 0.678982451 0.618 0.193 4.21E-07 Mast_cells RNASEK up RPS171 3.99E-12 0.678232058 0.539 0.148 2.09E-07 Mast_cells RPS17 up EIF4A11 8.04E-11 0.659280029 0.565 0.227 4.21E-06 Mast_cells EIF4A1 up MIF1 2.75E-11 0.65694838 0.568 0.159 1.44E-06 Mast_cells MIF up SELENOK 3.81E-16 0.648060583 0.8 0.511 1.99E-11 Mast_cells SELENOK up NDUFA13 2.92E-11 0.613751558 0.575 0.216 1.53E-06 Mast_cells NDUFA13 up WDR83OS 2.22E-10 0.599880454 0.48 0.125 1.16E-05 Mast_cells WDR83OS up CKLF 3.04E-10 0.571521344 0.628 0.318 1.59E-05 Mast_cells CKLF up RPL18A 8.52E-12 0.54478006 0.824 0.659 4.46E-07 Mast_cells RPL18A up C4orf481 2.21E-09 0.537969931 0.484 0.159 0.000115505 Mast_cells C4orf48 up ARL6IP41 2.10E-09 0.535951837 0.458 0.125 0.000110083 Mast_cells ARL6IP4 up HPGDS 3.05E-13 0.524692158 0.836 0.614 1.60E-08 Mast_cells HPGDS up KRTCAP2 6.06E-09 0.516767484 0.452 0.125 0.000317067 Mast_cells KRTCAP2 up LAT 1.24E-08 0.512868794 0.453 0.125 0.000646922 Mast_cells LAT up ARPC3 1.77E-11 0.494554066 0.78 0.5 9.26E-07 Mast_cells ARPC3 up DUSP2 5.21E-05 0.494523258 0.389 0.205 1 Mast_cells DUSP2 up TIMP1 9.13E-10 0.492155499 0.684 0.477 4.78E-05 Mast_cells TIMP1 up BST2 1.38E-08 0.481312892 0.585 0.33 0.000720702 Mast_cells BST2 up NEAT11 1.18E-09 0.480738876 0.795 0.602 6.18E-05 Mast_cells NEAT1 up CYBA 1.03E-07 0.480146951 0.583 0.295 0.00537711 Mast_cells CYBA up TOMM6 3.02E-08 0.479471768 0.407 0.091 0.001579764 Mast_cells TOMM6 up DDT 7.05E-08 0.475782571 0.362 0.091 0.003690036 Mast_cells DDT up NDUFC21 1.32E-07 0.469218942 0.455 0.193 0.00688898 Mast_cells NDUFC2 up SCGB2A15 8.36E-07 0.467657867 0.285 0.045 0.043764132 Mast_cells SCGB2A1 up MICOS10 7.02E-08 0.465896953 0.461 0.193 0.003674014 Mast_cells MICOS10 up UBE2S 3.06E-07 0.463022731 0.554 0.364 0.016030402 Mast_cells UBE2S up TSPO 2.15E-08 0.462580926 0.571 0.33 0.001127703 Mast_cells TSPO up HIST1H4C1 9.12E-07 0.455501321 0.503 0.261 0.047739361 Mast_cells HIST1H4C up ARPC1B 4.24E-07 0.455212904 0.471 0.205 0.022222924 Mast_cells ARPC1B up ATP5F1D 2.08E-07 0.443301448 0.532 0.273 0.010914375 Mast_cells ATP5F1D up S100A10 4.38E-07 0.442135056 0.541 0.307 0.022911217 Mast_cells S100A10 up SERTAD1 2.18E-06 0.439785582 0.546 0.307 0.114209886 Mast_cells SERTAD1 up S100A6 3.76E-20 0.437214604 0.955 0.932 1.97E-15 Mast_cells S100A6 up SELENOH 4.87E-07 0.434597192 0.444 0.17 0.025510246 Mast_cells SELENOH up WFDC25 2.59E-05 0.428194213 0.3 0.102 1 Mast_cells WFDC2 up MT2A1 0.000354175 0.419645192 0.451 0.273 1 Mast_cells MT2A up GNG5 7.83E-07 0.418074957 0.457 0.159 0.041014357 Mast_cells GNG5 up RPL36AL 9.43E-12 0.416121384 0.845 0.705 4.94E-07 Mast_cells RPL36AL up ARHGAP181 1.52E-07 0.415848838 0.624 0.398 0.007968874 Mast_cells ARHGAP18 up POLD4 2.58E-06 0.415317369 0.362 0.125 0.134974271 Mast_cells POLD4 up C9orf16 1.75E-06 0.412284092 0.482 0.239 0.091378566 Mast_cells C9orf16 up FAU 2.92E-15 0.407268982 0.952 0.864 1.53E-10 Mast_cells FAU up CD69 6.25E-06 0.406797007 0.756 0.534 0.327201961 Mast_cells CD69 up PHLDA2 2.00E-05 0.406340667 0.328 0.114 1 Mast_cells PHLDA2 up CLIC1 8.42E-13 0.405431988 0.874 0.75 4.41E-08 Mast_cells CLIC1 up HPGD 5.94E-11 0.402748846 0.917 0.841 3.11E-06 Mast_cells HPGD up IDS 1.15E-05 0.398853865 0.48 0.239 0.60305759 Mast_cells IDS up CD37 2.60E-09 0.39768828 0.8 0.591 0.000136267 Mast_cells CD37 up UQCR11 4.95E-06 0.397458283 0.533 0.307 0.259262097 Mast_cells UQCR11 up SAMSN1 1.41E-08 0.395084434 0.822 0.602 0.000740377 Mast_cells SAMSN1 up UBXN1 3.83E-06 0.389530927 0.494 0.273 0.200376201 Mast_cells UBXN1 up VPS28 9.21E-07 0.386207349 0.603 0.375 0.048245314 Mast_cells VPS28 up NDUFA11 1.01E-05 0.383642816 0.482 0.261 0.529524217 Mast_cells NDUFA11 up TYROBP 2.94E-08 0.381233581 0.791 0.625 0.001538353 Mast_cells TYROBP up VAMP8 2.01E-06 0.380465922 0.6 0.386 0.10539572 Mast_cells VAMP8 up UXT 6.74E-06 0.378333985 0.449 0.239 0.352766071 Mast_cells UXT up GPX4 5.30E-08 0.377657276 0.735 0.58 0.002774177 Mast_cells GPX4 up RPL10 6.48E-16 0.375770386 0.959 0.977 3.39E-11 Mast_cells RPL10 up HLA-C1 4.59E-08 0.375116441 0.781 0.625 0.002401597 Mast_cells HLA-C up LDHB 2.11E-06 0.372285476 0.633 0.443 0.110342974 Mast_cells LDHB up BCL2A1 0.000155948 0.371342716 0.329 0.136 1 Mast_cells BCL2A1 up FCER1G 7.93E-11 0.369944693 0.912 0.795 4.15E-06 Mast_cells FCER1G up FXYD5 3.29E-07 0.368822419 0.84 0.614 0.017251545 Mast_cells FXYD5 up RABAC1 2.59E-05 0.367800652 0.467 0.261 1 Mast_cells RABAC1 up COX4I1 1.69E-09 0.366966772 0.82 0.659 8.83E-05 Mast_cells COX4I1 up CRIP1 8.89E-05 0.36585427 0.305 0.114 1 Mast_cells CRIP1 up CD83 8.86E-07 0.36262733 0.732 0.614 0.046375704 Mast_cells CD83 up ATP6V1G1 3.86E-06 0.361571452 0.601 0.409 0.2023308 Mast_cells ATP6V1G1 up ABRACL 2.92E-05 0.360812091 0.389 0.182 1 Mast_cells ABRACL up TMEM176B 1.99E-05 0.360330839 0.563 0.409 1 Mast_cells TMEM176B up RGS10 2.04E-06 0.358909754 0.679 0.534 0.107029508 Mast_cells RGS10 up C17orf49 9.84E-06 0.358574817 0.301 0.08 0.515324412 Mast_cells C17orf49 up AC020916.1 3.66E-05 0.356853421 0.61 0.432 1 Mast_cells AC020916.1 up T H U M P D 3 - AS1 2.09E-05 0.356149357 0.338 0.125 1 Mast_cells T H U M P D 3 - AS1 up COX17 5.61E-05 0.353406594 0.462 0.284 1 Mast_cells COX17 up IL18 7.06E-05 0.352697603 0.402 0.216 1 Mast_cells IL18 up C18orf32 1.41E-05 0.351663469 0.316 0.102 0.740669802 Mast_cells C18orf32 up SCAND1 2.09E-05 0.349769571 0.304 0.102 1 Mast_cells SCAND1 up TRAPPC5 1.25E-05 0.348480914 0.312 0.091 0.656892713 Mast_cells TRAPPC5 up S100A41 3.17E-11 0.347089274 0.939 0.841 1.66E-06 Mast_cells S100A4 up BIRC3 0.000193657 0.344336921 0.566 0.443 1 Mast_cells BIRC3 up SRP14 3.03E-09 0.34402443 0.831 0.727 0.000158499 Mast_cells SRP14 up NDUFV2 1.22E-05 0.343390073 0.297 0.08 0.636231689 Mast_cells NDUFV2 up CIAO2B 5.75E-05 0.341373455 0.44 0.261 1 Mast_cells CIAO2B up SPNS1 2.00E-05 0.341230933 0.249 0.057 1 Mast_cells SPNS1 up GABARAPL2 3.28E-05 0.341147088 0.488 0.307 1 Mast_cells GABARAPL2 up NACA 1.63E-15 0.339587878 0.954 0.864 8.56E-11 Mast_cells NACA up RPL22 2.87E-10 0.339441847 0.866 0.795 1.50E-05 Mast_cells RPL22 up GTF2B 0.00015292 0.339258055 0.373 0.193 1 Mast_cells GTF2B up DNAJB6 4.60E-05 0.336670505 0.612 0.5 1 Mast_cells DNAJB6 up COX5B 1.53E-05 0.334743099 0.599 0.398 0.80369622 Mast_cells COX5B up SKP1 7.69E-08 0.333268243 0.728 0.636 0.004028912 Mast_cells SKP1 up RPS15 7.13E-13 0.329974608 0.913 0.92 3.73E-08 Mast_cells RPS15 up RPL22L1 0.000111277 0.32960816 0.482 0.295 1 Mast_cells RPL22L1 up RPL24 7.61E-13 0.325108484 0.929 0.898 3.98E-08 Mast_cells RPL24 up EDF1 1.03E-05 0.322107007 0.667 0.534 0.536796435 Mast_cells EDF1 up ZFAS1 7.58E-06 0.322084558 0.685 0.58 0.39696074 Mast_cells ZFAS1 up PSME1 9.17E-05 0.32192201 0.53 0.364 1 Mast_cells PSME1 up IER2 8.53E-08 0.321873318 0.876 0.841 0.004468597 Mast_cells IER2 up RHEX 7.08E-07 0.321558937 0.817 0.682 0.037075004 Mast_cells RHEX up RBM4 9.95E-05 0.321223481 0.356 0.159 1 Mast_cells RBM4 up MT1X1 0.008945435 0.320823969 0.208 0.102 1 Mast_cells MT1X up DYNLL1 5.06E-07 0.31704905 0.784 0.67 0.026497738 Mast_cells DYNLL1 up IGFLR1 0.000104977 0.316625356 0.269 0.091 1 Mast_cells IGFLR1 up CKS2 0.000818282 0.316546282 0.438 0.284 1 Mast_cells CKS2 up FTL 1.14E-17 0.314372979 0.972 0.989 5.96E-13 Mast_cells FTL up TMBIM41 0.000134593 0.312536437 0.443 0.239 1 Mast_cells TMBIM4 up CAPG 2.73E-07 0.311226648 0.875 0.773 0.014309866 Mast_cells CAPG up RPL26 2.64E-13 0.31021697 0.959 0.955 1.38E-08 Mast_cells RPL26 up MRPS24 2.55E-05 0.308583666 0.234 0.045 1 Mast_cells MRPS24 up RPL7A 1.18E-12 0.307166183 0.933 0.92 6.19E-08 Mast_cells RPL7A up SNHG8 2.86E-05 0.305447863 0.627 0.5 1 Mast_cells SNHG8 up PSMB10 5.01E-05 0.304505957 0.259 0.068 1 Mast_cells PSMB10 up TNF 0.004527583 0.304103235 0.29 0.159 1 Mast_cells TNF up NDUFB8 0.000241547 0.303948331 0.427 0.261 1 Mast_cells NDUFB8 up COX6A1 5.01E-05 0.303737126 0.592 0.443 1 Mast_cells COX6A1 up GIHCG 0.000231305 0.303173396 0.26 0.091 1 Mast_cells GIHCG up PRG2 0.000989647 0.302913215 0.184 0.045 1 Mast_cells PRG2 up NEDD8 0.0001628 0.302452884 0.535 0.409 1 Mast_cells NEDD8 up RPS26 1.07E-07 0.301712438 0.798 0.705 0.005586528 Mast_cells RPS26 up DNAJB11 6.89E-07 0.301024361 0.837 0.795 0.036094734 Mast_cells DNAJB1 up PRDX1 1.41E-06 0.299616377 0.784 0.727 0.073696864 Mast_cells PRDX1 up FTH1 1.39E-21 0.296043119 0.998 1 7.29E-17 Mast_cells FTH1 up TOMM5 0.000131379 0.295970645 0.239 0.068 1 Mast_cells TOMM5 up UBB 2.92E-16 0.295868623 0.973 0.989 1.53E-11 Mast_cells UBB up BTG1 2.02E-07 0.294780663 0.845 0.761 0.01057497 Mast_cells BTG1 up TMEM176A 0.000654189 0.292187098 0.365 0.205 1 Mast_cells TMEM176A up ANXA4 0.000462931 0.292053537 0.511 0.352 1 Mast_cells ANXA4 up ELOB 4.37E-05 0.291914456 0.644 0.489 1 Mast_cells ELOB up MRPL18 0.001574517 0.286476983 0.361 0.227 1 Mast_cells MRPL18 up FAM133B 0.001348083 0.286304697 0.37 0.227 1 Mast_cells FAM133B up PDCD6 0.000192158 0.286241521 0.274 0.091 1 Mast_cells PDCD6 up FIS1 0.000368837 0.286227157 0.482 0.33 1 Mast_cells FIS1 up ATP5MG 1.09E-05 0.285238721 0.697 0.614 0.568638773 Mast_cells ATP5MG up TSTD1 0.001066893 0.283641788 0.364 0.216 1 Mast_cells TSTD1 up MALAT1 1.91E-15 0.283384386 0.999 1 1.00E-10 Mast_cells MALAT1 up BEX4 0.0003394 0.283331397 0.509 0.341 1 Mast_cells BEX4 up LYL1 0.000666826 0.283217847 0.363 0.182 1 Mast_cells LYL1 up DRAP1 0.000234717 0.283107104 0.549 0.409 1 Mast_cells DRAP1 up PET100 0.000423021 0.282044091 0.323 0.148 1 Mast_cells PET100 up SUPT4H1 0.000225787 0.281645766 0.25 0.08 1 Mast_cells SUPT4H1 up NDUFB7 0.00056874 0.281365539 0.37 0.205 1 Mast_cells NDUFB7 up SAT1 6.01E-09 0.27943347 0.911 0.886 0.000314551 Mast_cells SAT1 up NDUFA4 1.39E-06 0.279128123 0.786 0.705 0.072763047 Mast_cells NDUFA4 up MPG 0.00028019 0.279120649 0.304 0.125 1 Mast_cells MPG up ANKRD28 0.001831529 0.278329292 0.452 0.318 1 Mast_cells ANKRD28 up ANAPC16 0.00084276 0.277919585 0.447 0.307 1 Mast_cells ANAPC16 up DBI 0.00017639 0.276477517 0.617 0.534 1 Mast_cells DBI up PYCARD 0.000279825 0.276218284 0.433 0.239 1 Mast_cells PYCARD up HIGD2A 0.001409671 0.274778879 0.403 0.273 1 Mast_cells HIGD2A up GPR65 0.003152197 0.274104393 0.452 0.318 1 Mast_cells GPR65 up ATP5F1C 0.000328326 0.273421245 0.555 0.443 1 Mast_cells ATP5F1C up DNAJA1 2.71E-09 0.272627033 0.915 0.875 0.000141714 Mast_cells DNAJA1 up PSMA7 0.000165573 0.271742604 0.632 0.534 1 Mast_cells PSMA7 up PPP1R2 0.002596617 0.269849032 0.369 0.25 1 Mast_cells PPP1R2 up A1BG 7.08E-05 0.269193355 0.192 0.023 1 Mast_cells A1BG up ATP6V0B 0.000439277 0.26908942 0.578 0.455 1 Mast_cells ATP6V0B up CCDC59 0.002196176 0.26853506 0.421 0.295 1 Mast_cells CCDC59 up NXT1 0.00147742 0.267127146 0.364 0.216 1 Mast_cells NXT1 up SPINT2 0.001070765 0.266769506 0.444 0.284 1 Mast_cells SPINT2 up COX7A2 0.00035358 0.264940659 0.597 0.5 1 Mast_cells COX7A2 up ANAPC11 0.001597411 0.264450649 0.363 0.227 1 Mast_cells ANAPC11 up OSTF1 0.000883194 0.264400298 0.326 0.159 1 Mast_cells OSTF1 up LGALS31 5.40E-05 0.264366812 0.732 0.682 1 Mast_cells LGALS3 up UBE2V1 0.000659631 0.261381282 0.274 0.114 1 Mast_cells UBE2V1 up CST7 0.002395214 0.260968956 0.344 0.193 1 Mast_cells CST7 up CST3 2.06E-05 0.26073065 0.727 0.659 1 Mast_cells CST3 up NDST2 0.002145999 0.260586708 0.259 0.125 1 Mast_cells NDST2 up PSMA2 0.001809194 0.260342258 0.365 0.227 1 Mast_cells PSMA2 up GPS2 0.000427366 0.259696482 0.224 0.068 1 Mast_cells GPS2 up SNX6 0.002821064 0.258378088 0.36 0.239 1 Mast_cells SNX6 up NDUFB10 0.00113105 0.258181558 0.463 0.307 1 Mast_cells NDUFB10 up CHMP4A 0.000428414 0.257213045 0.244 0.08 1 Mast_cells CHMP4A up PSMA1 0.00179737 0.256420047 0.336 0.193 1 Mast_cells PSMA1 up G3BP2 0.001394803 0.255595653 0.568 0.443 1 Mast_cells G3BP2 up RPL28 3.40E-12 0.255547816 0.961 0.955 1.78E-07 Mast_cells RPL28 up AGTRAP 0.001225202 0.255335743 0.356 0.182 1 Mast_cells AGTRAP up ATP6V1F 0.000229056 0.255234452 0.698 0.58 1 Mast_cells ATP6V1F up SUB1 6.18E-06 0.254886855 0.791 0.716 0.323329968 Mast_cells SUB1 up CD91 2.71E-06 0.254758409 0.824 0.773 0.142034076 Mast_cells CD9 up COX16 0.000321342 0.251398441 0.198 0.045 1 Mast_cells COX16 up NUTM2A-AS1 0.001418012 0.250900581 0.285 0.136 1 Mast_cells NUTM2A-AS1 up NDUFA71 0.000200322 0.250866518 0.209 0.045 1 Mast_cells NDUFA7 up NFKBIA 7.55E-12 0.250861623 0.965 0.943 3.95E-07 Mast_cells NFKBIA up AL157895.1 0.000500763 0.250387471 0.643 0.568 1 Mast_cells AL157895.1 up NME3 0.001478136 0.250185793 0.318 0.17 1 Mast_cells NME3 up PAPSS2 1.65E-12 -0.250095673 0.057 0.261 8.66E-08 Mast_cells PAPSS2 down FOXL2NB 6.82E-13 -0.250167051 0.056 0.261 3.57E-08 Mast_cells FOXL2NB down IFITM2 9.49E-07 -0.250202117 0.765 0.909 0.049677547 Mast_cells IFITM2 down RAI1 3.24E-06 -0.250824524 0.113 0.284 0.169570873 Mast_cells RAI1 down ZBTB38 6.68E-05 -0.25086274 0.266 0.477 1 Mast_cells ZBTB38 down CTTN 2.22E-08 -0.250865864 0.114 0.318 0.001164256 Mast_cells CTTN down CAPZA1 0.000175077 -0.251072473 0.353 0.545 1 Mast_cells CAPZA1 down ACTN1 8.72E-07 -0.251075046 0.117 0.295 0.045658513 Mast_cells ACTN1 down RERE 0.000120046 -0.251091841 0.295 0.489 1 Mast_cells RERE down FADS2 1.71E-07 -0.251149527 0.106 0.295 0.00894561 Mast_cells FADS2 down TOB2 1.36E-05 -0.251281672 0.24 0.466 0.711016982 Mast_cells TOB2 down SMARCC1 5.28E-06 -0.251666435 0.233 0.466 0.276360516 Mast_cells SMARCC1 down DDX42 6.10E-05 -0.251823062 0.2 0.375 1 Mast_cells DDX42 down NORAD 0.000113105 -0.251877239 0.481 0.682 1 Mast_cells NORAD down SP1 1.07E-07 -0.251882016 0.108 0.307 0.005612604 Mast_cells SP1 down JAZF1 3.66E-07 -0.25196512 0.165 0.398 0.019181209 Mast_cells JAZF1 down TBC1D5 3.64E-10 -0.252019023 0.123 0.375 1.90E-05 Mast_cells TBC1D5 down VEGFA 0.000467551 -0.252658513 0.45 0.67 1 Mast_cells VEGFA down PABPC1 3.33E-09 -0.253096835 0.874 0.977 0.000174507 Mast_cells PABPC1 down TPM2 6.08E-07 -0.253500677 0.174 0.409 0.031829192 Mast_cells TPM2 down NONO 0.000211669 -0.253876093 0.387 0.602 1 Mast_cells NONO down ARHGAP1 5.01E-06 -0.253945311 0.122 0.295 0.262392258 Mast_cells ARHGAP1 down MACF1 0.000161703 -0.254000658 0.317 0.511 1 Mast_cells MACF1 down BTBD3 2.60E-11 -0.254254871 0.064 0.261 1.36E-06 Mast_cells BTBD3 down SCAP 4.74E-07 -0.25434558 0.075 0.227 0.024813149 Mast_cells SCAP down C16orf72 4.97E-06 -0.254470349 0.187 0.398 0.260414643 Mast_cells C16orf72 down EGR11 7.23E-07 -0.254587174 0.855 0.932 0.037837379 Mast_cells EGR1 down LSM14A 1.36E-05 -0.254635456 0.295 0.534 0.713521574 Mast_cells LSM14A down ADGRA2 2.05E-09 -0.254663394 0.096 0.307 0.000107418 Mast_cells ADGRA2 down MT-TF 1.73E-11 -0.254763173 0.073 0.295 9.05E-07 Mast_cells MT-TF down LCOR 4.13E-08 -0.254843213 0.101 0.295 0.00216201 Mast_cells LCOR down LRRC58 1.34E-05 -0.254956319 0.11 0.261 0.703950679 Mast_cells LRRC58 down RAB1A 0.000207835 -0.254976672 0.358 0.545 1 Mast_cells RAB1A down PHLDB1 2.26E-08 -0.255686875 0.1 0.295 0.001182298 Mast_cells PHLDB1 down MT-ND2 1.62E-16 -0.255738085 0.994 1 8.47E-12 Mast_cells MT-ND2 down CYB5R3 3.92E-05 -0.255799683 0.304 0.534 1 Mast_cells CYB5R3 down PCYOX1 1.81E-06 -0.256334284 0.155 0.352 0.094928965 Mast_cells PCYOX1 down PDLIM1 3.34E-07 -0.256386788 0.091 0.261 0.017492455 Mast_cells PDLIM1 down RPS13P2 1.32E-10 -0.256523012 0.047 0.216 6.89E-06 Mast_cells RPS13P2 down AR 1.38E-05 -0.256807574 0.139 0.307 0.723167569 Mast_cells AR down VASN 6.02E-09 -0.256811207 0.078 0.261 0.000315062 Mast_cells VASN down DUSP8 4.49E-08 -0.257082216 0.094 0.284 0.002350064 Mast_cells DUSP8 down SNX1 2.57E-06 -0.258110415 0.163 0.364 0.134456427 Mast_cells SNX1 down MT-ND1 1.81E-20 -0.258386097 0.99 1 9.49E-16 Mast_cells MT-ND1 down ADAM33 1.91E-08 -0.258514322 0.087 0.273 0.000998312 Mast_cells ADAM33 down CDK16 9.36E-10 -0.258639317 0.077 0.273 4.90E-05 Mast_cells CDK16 down HNRNPM 0.000109997 -0.259182442 0.545 0.773 1 Mast_cells HNRNPM down GNLY1 3.91E-09 -0.259299734 0.03 0.159 0.000204959 Mast_cells GNLY down CCN1 6.19E-06 -0.259351465 0.156 0.352 0.323938804 Mast_cells CCN1 down MFGE8 1.46E-06 -0.259409016 0.145 0.341 0.07666062 Mast_cells MFGE8 down MAP7D1 5.88E-06 -0.259451057 0.208 0.42 0.307688447 Mast_cells MAP7D1 down GALNT1 7.63E-06 -0.260017246 0.194 0.398 0.399315348 Mast_cells GALNT1 down SLC25A37 1.39E-07 -0.26003813 0.125 0.33 0.007257633 Mast_cells SLC25A37 down SPTAN1 4.50E-06 -0.260116288 0.23 0.455 0.235526403 Mast_cells SPTAN1 down C14orf132 2.49E-08 -0.260155681 0.094 0.284 0.00130588 Mast_cells C14orf132 down EMC10 6.16E-06 -0.260224379 0.275 0.511 0.322690437 Mast_cells EMC10 down MAGT1 5.25E-06 -0.260234164 0.177 0.375 0.274832681 Mast_cells MAGT1 down TWSG1 1.31E-08 -0.260256878 0.111 0.318 0.000683392 Mast_cells TWSG1 down PTEN 6.67E-06 -0.260351741 0.248 0.477 0.349242139 Mast_cells PTEN down RELA 5.64E-06 -0.260461171 0.248 0.489 0.295089099 Mast_cells RELA down MAP1B 4.11E-08 -0.26073283 0.124 0.341 0.002153747 Mast_cells MAP1B down PDS5B 5.38E-06 -0.260911502 0.177 0.375 0.281641647 Mast_cells PDS5B down ABL1 5.33E-07 -0.261047427 0.121 0.307 0.027926451 Mast_cells ABL1 down GPX1 1.60E-08 -0.261068729 0.099 0.307 0.00083707 Mast_cells GPX1 down CTSK 2.02E-06 -0.261353797 0.146 0.341 0.105715002 Mast_cells CTSK down TAF6 2.93E-09 -0.261861178 0.104 0.318 0.000153456 Mast_cells TAF6 down EP300 1.28E-05 -0.262175172 0.252 0.466 0.66997559 Mast_cells EP300 down ACTR2 0.000190835 -0.262500284 0.443 0.67 1 Mast_cells ACTR2 down TRAM1 3.33E-05 -0.262643868 0.312 0.534 1 Mast_cells TRAM1 down FAM120A 1.02E-05 -0.262821271 0.262 0.5 0.533627437 Mast_cells FAM120A down SNORD3A 1.24E-09 -0.262966591 0.065 0.25 6.50E-05 Mast_cells SNORD3A down CLIP3 2.48E-14 -0.263054584 0.055 0.273 1.30E-09 Mast_cells CLIP3 down SBNO1 7.14E-06 -0.263809484 0.144 0.318 0.373627367 Mast_cells SBNO1 down SEC13 8.52E-07 -0.264143356 0.186 0.409 0.04459087 Mast_cells SEC13 down RANBP2 5.85E-05 -0.26465348 0.269 0.466 1 Mast_cells RANBP2 down HNRNPR 5.38E-05 -0.264780384 0.436 0.659 1 Mast_cells HNRNPR down DYNC1H1 1.89E-05 -0.264993171 0.256 0.466 0.990218061 Mast_cells DYNC1H1 down KDM5C 1.21E-06 -0.265099547 0.173 0.386 0.063189576 Mast_cells KDM5C down TMEM214 7.47E-07 -0.265178151 0.128 0.318 0.03910244 Mast_cells TMEM214 down NUTF2 1.69E-05 -0.265269083 0.227 0.432 0.884212148 Mast_cells NUTF2 down NXF1 1.52E-07 -0.265294769 0.182 0.432 0.007980174 Mast_cells NXF1 down EMX2 1.01E-06 -0.265444879 0.145 0.341 0.052680226 Mast_cells EMX2 down CCBE1 9.22E-08 -0.265452257 0.094 0.273 0.004825815 Mast_cells CCBE1 down JMJD1C 0.000265188 -0.26557288 0.399 0.602 1 Mast_cells JMJD1C down WLS 1.56E-06 -0.265611489 0.123 0.295 0.081907529 Mast_cells WLS down CLSTN1 7.25E-07 -0.265649423 0.16 0.364 0.037982774 Mast_cells CLSTN1 down EFEMP2 8.36E-08 -0.265965803 0.137 0.352 0.004376173 Mast_cells EFEMP2 down EI24 1.64E-06 -0.26613224 0.178 0.386 0.085969078 Mast_cells EI24 down WBP11 4.31E-05 -0.266286373 0.346 0.58 1 Mast_cells WBP11 down DAXX 5.39E-08 -0.266357657 0.109 0.307 0.002822906 Mast_cells DAXX down ZC3HAV1 5.13E-06 -0.266371999 0.252 0.489 0.268627636 Mast_cells ZC3HAV1 down EIF4A1P10 1.45E-11 -0.266698571 0.074 0.295 7.61E-07 Mast_cells EIF4A1P10 down NT5DC2 1.78E-05 -0.268127014 0.221 0.42 0.933657918 Mast_cells NT5DC2 down NCOR2 0.000161608 -0.268165019 0.293 0.477 1 Mast_cells NCOR2 down CARM1 6.04E-10 -0.268678489 0.098 0.318 3.16E-05 Mast_cells CARM1 down RHOQ 1.54E-07 -0.26871449 0.171 0.409 0.008056 Mast_cells RHOQ down STIM1 7.88E-09 -0.268733949 0.13 0.364 0.000412329 Mast_cells STIM1 down ANK2 5.51E-11 -0.268969457 0.047 0.216 2.89E-06 Mast_cells ANK2 down COPB2 6.95E-06 -0.26939141 0.274 0.511 0.363700344 Mast_cells COPB2 down RAD23B 2.82E-05 -0.269646678 0.304 0.523 1 Mast_cells RAD23B down PITPNA 3.89E-09 -0.269763648 0.117 0.341 0.000203444 Mast_cells PITPNA down TRIP6 7.20E-10 -0.269824001 0.106 0.33 3.77E-05 Mast_cells TRIP6 down AGO2 1.58E-07 -0.270011594 0.115 0.307 0.008270053 Mast_cells AGO2 down GABARAPL1 4.87E-06 -0.270475429 0.199 0.409 0.25484613 Mast_cells GABARAPL1 down RPN1 2.77E-05 -0.270499594 0.217 0.409 1 Mast_cells RPN1 down RBM25 3.69E-05 -0.271150633 0.515 0.727 1 Mast_cells RBM25 down IPO7 1.11E-07 -0.271202219 0.184 0.432 0.005789525 Mast_cells IPO7 down TRIM56 2.08E-08 -0.271370572 0.137 0.364 0.001088138 Mast_cells TRIM56 down PTPN14 1.29E-19 -0.271480177 0.04 0.273 6.74E-15 Mast_cells PTPN14 down MEIS1 6.50E-09 -0.271700281 0.097 0.295 0.000340344 Mast_cells MEIS1 down STAT6 1.86E-08 -0.271730661 0.109 0.318 0.000971993 Mast_cells STAT6 down TPBG 1.69E-09 -0.272081168 0.121 0.352 8.87E-05 Mast_cells TPBG down CLTC 1.01E-05 -0.272867524 0.26 0.489 0.527534419 Mast_cells CLTC down COPG1 5.32E-08 -0.272908006 0.143 0.364 0.002785853 Mast_cells COPG1 down APOD 3.76E-10 -0.273554177 0.1 0.33 1.97E-05 Mast_cells APOD down KANK2 3.69E-06 -0.274265029 0.237 0.477 0.193299164 Mast_cells KANK2 down MED1 2.14E-08 -0.274557696 0.106 0.307 0.00112183 Mast_cells MED1 down PEA15 7.25E-08 -0.274576132 0.156 0.386 0.003798149 Mast_cells PEA15 down TUNAR1 2.96E-12 -0.274625051 0.05 0.239 1.55E-07 Mast_cells TUNAR down BRD2 7.55E-05 -0.274776697 0.553 0.761 1 Mast_cells BRD2 down MARVELD1 8.54E-08 -0.275348016 0.114 0.307 0.004469968 Mast_cells MARVELD1 down NFE2L1 2.76E-08 -0.276117683 0.18 0.443 0.001446277 Mast_cells NFE2L1 down SEPTIN9 4.14E-06 -0.276141605 0.358 0.648 0.217021865 Mast_cells SEPTIN9 down RAP2B 1.40E-07 -0.276240073 0.163 0.398 0.007304849 Mast_cells RAP2B down PRKCSH 3.77E-06 -0.276293064 0.252 0.489 0.19724617 Mast_cells PRKCSH down TNC1 4.35E-10 -0.276807666 0.074 0.273 2.28E-05 Mast_cells TNC down TCEAL9 7.04E-08 -0.276862742 0.156 0.386 0.003686565 Mast_cells TCEAL9 down PRXL2A 1.64E-05 -0.276974879 0.183 0.364 0.857937972 Mast_cells PRXL2A down EIF4G1 1.69E-06 -0.277421899 0.209 0.432 0.088335217 Mast_cells EIF4G1 down TIAL1 3.15E-06 -0.277496638 0.237 0.466 0.16485676 Mast_cells TIAL1 down PSME3 1.12E-08 -0.277667658 0.146 0.386 0.000588844 Mast_cells PSME3 down ARHGEF40 1.25E-06 -0.279078239 0.215 0.455 0.065218898 Mast_cells ARHGEF40 down UBXN7 5.60E-07 -0.279745769 0.107 0.284 0.029316979 Mast_cells UBXN7 down MAFF1 0.000136436 -0.279863294 0.275 0.466 1 Mast_cells MAFF down MAN1A2 5.11E-07 -0.280700176 0.189 0.42 0.026772442 Mast_cells MAN1A2 down RAB1B 3.76E-07 -0.280895977 0.206 0.455 0.019677002 Mast_cells RAB1B down TEAD2 4.95E-10 -0.281091703 0.082 0.284 2.59E-05 Mast_cells TEAD2 down CREB3L2 1.52E-05 -0.281129049 0.259 0.477 0.79572524 Mast_cells CREB3L2 down WDR82 7.79E-06 -0.281284158 0.226 0.432 0.407917534 Mast_cells WDR82 down EIF2S3 3.08E-05 -0.281323875 0.493 0.727 1 Mast_cells EIF2S3 down SPN 1.25E-05 -0.281421468 0.144 0.318 0.652797059 Mast_cells SPN down APOBEC3C 7.54E-06 -0.281604138 0.261 0.5 0.394672505 Mast_cells APOBEC3C down ACTN4 4.31E-05 -0.281671693 0.476 0.682 1 Mast_cells ACTN4 down CLEC11A 1.08E-07 -0.281760266 0.158 0.386 0.005664674 Mast_cells CLEC11A down CTSB 4.72E-05 -0.282389756 0.529 0.773 1 Mast_cells CTSB down RNF10 3.21E-07 -0.282577221 0.207 0.455 0.016792872 Mast_cells RNF10 down EPN1 5.18E-07 -0.282660192 0.16 0.364 0.02712304 Mast_cells EPN1 down CERS2 3.57E-06 -0.282917575 0.26 0.5 0.186736108 Mast_cells CERS2 down SDC3 1.18E-10 -0.283086538 0.1 0.33 6.16E-06 Mast_cells SDC3 down PRICKLE2 9.84E-12 -0.283103066 0.077 0.295 5.15E-07 Mast_cells PRICKLE2 down ABCG1 4.32E-12 -0.283584801 0.053 0.25 2.26E-07 Mast_cells ABCG1 down ITGA5 4.76E-13 -0.283609736 0.083 0.33 2.49E-08 Mast_cells ITGA5 down ARF1 9.11E-07 -0.283823447 0.668 0.875 0.047705006 Mast_cells ARF1 down MTSS2 1.36E-15 -0.283908438 0.043 0.25 7.13E-11 Mast_cells MTSS2 down TEAD1 1.16E-10 -0.284005397 0.101 0.33 6.08E-06 Mast_cells TEAD1 down FKBP10 3.62E-07 -0.284019097 0.146 0.352 0.018957551 Mast_cells FKBP10 down TCF7L2 2.37E-08 -0.284044773 0.108 0.307 0.001238937 Mast_cells TCF7L2 down RCC2 6.68E-08 -0.284472718 0.175 0.42 0.003497464 Mast_cells RCC2 down AL355309.1 8.11E-13 -0.284654531 0.062 0.284 4.24E-08 Mast_cells AL355309.1 down ELMSAN1 2.03E-07 -0.284797724 0.164 0.386 0.01060782 Mast_cells ELMSAN1 down MAVS 1.57E-09 -0.285028658 0.103 0.318 8.23E-05 Mast_cells MAVS down FJX1 1.51E-09 -0.285116213 0.095 0.307 7.88E-05 Mast_cells FJX1 down COPA 2.86E-05 -0.285440322 0.31 0.511 1 Mast_cells COPA down ERN1 1.45E-07 -0.285580735 0.157 0.375 0.007598147 Mast_cells ERN1 down PGBD5 9.86E-08 -0.285903485 0.138 0.352 0.005162554 Mast_cells PGBD5 down TSKU 6.62E-10 -0.286387997 0.094 0.307 3.47E-05 Mast_cells TSKU down RND3 7.51E-07 -0.286515437 0.128 0.318 0.039325495 Mast_cells RND3 down PUM1 1.51E-06 -0.286584101 0.239 0.477 0.078892719 Mast_cells PUM1 down MTND2P28 6.82E-09 -0.286850561 0.114 0.341 0.000356852 Mast_cells MTND2P28 down SELENOP2 5.87E-07 -0.287143134 0.178 0.398 0.030734074 Mast_cells SELENOP down SMOC2 6.69E-11 -0.287157407 0.067 0.261 3.50E-06 Mast_cells SMOC2 down EIF4A2 5.38E-06 -0.287189338 0.666 0.886 0.281702783 Mast_cells EIF4A2 down RPL13AP5 2.08E-17 -0.288153495 0.03 0.227 1.09E-12 Mast_cells RPL13AP5 down RBPMS 3.61E-08 -0.288624849 0.122 0.33 0.001888666 Mast_cells RBPMS down ADD1 2.67E-06 -0.288787849 0.289 0.545 0.139891903 Mast_cells ADD1 down ABHD2 3.61E-06 -0.28984042 0.221 0.443 0.188822102 Mast_cells ABHD2 down BMP1 1.65E-09 -0.290034473 0.123 0.352 8.63E-05 Mast_cells BMP1 down EIF2AK2 3.23E-07 -0.290272617 0.216 0.466 0.016893928 Mast_cells EIF2AK2 down CD164 4.27E-06 -0.290487729 0.343 0.614 0.223611532 Mast_cells CD164 down TUBB 5.82E-06 -0.290968994 0.536 0.784 0.304621052 Mast_cells TUBB down EEF2 1.11E-07 -0.291050746 0.737 0.943 0.005809769 Mast_cells EEF2 down CFAP20 4.45E-08 -0.291091226 0.159 0.398 0.002329947 Mast_cells CFAP20 down CLPTM1 1.08E-07 -0.292069697 0.17 0.398 0.005678294 Mast_cells CLPTM1 down ARF6 5.20E-06 -0.292305824 0.485 0.761 0.272033911 Mast_cells ARF6 down CEBPB 5.23E-05 -0.292656664 0.391 0.591 1 Mast_cells CEBPB down BCL9L 1.26E-11 -0.292836757 0.077 0.295 6.61E-07 Mast_cells BCL9L down GPI 2.11E-05 -0.292943566 0.197 0.375 1 Mast_cells GPI down PGD 4.61E-07 -0.29311203 0.201 0.432 0.024130481 Mast_cells PGD down AC024293.1 7.55E-10 -0.293360212 0.097 0.318 3.95E-05 Mast_cells AC024293.1 down PTMAP5 1.15E-09 -0.293431046 0.107 0.341 6.00E-05 Mast_cells PTMAP5 down TGM21 2.07E-06 -0.293820797 0.128 0.307 0.108299839 Mast_cells TGM2 down PTBP3 3.56E-06 -0.294423653 0.265 0.511 0.186378678 Mast_cells PTBP3 down ZCCHC24 4.09E-07 -0.294729217 0.122 0.307 0.021440817 Mast_cells ZCCHC24 down BSDC1 4.80E-09 -0.294776395 0.168 0.432 0.000251336 Mast_cells BSDC1 down GRINA 8.57E-08 -0.295009151 0.205 0.466 0.004487259 Mast_cells GRINA down KLF9 7.39E-08 -0.296013229 0.118 0.318 0.003871314 Mast_cells KLF9 down COL15A1 7.62E-11 -0.296093885 0.075 0.284 3.99E-06 Mast_cells COL15A1 down QSOX1 9.61E-11 -0.296260701 0.104 0.341 5.03E-06 Mast_cells QSOX1 down ARID1A 4.88E-06 -0.296300505 0.288 0.523 0.255442553 Mast_cells ARID1A down HNRNPL 5.91E-06 -0.296376743 0.305 0.545 0.309651448 Mast_cells HNRNPL down EIF4G3 1.05E-06 -0.296447044 0.199 0.42 0.055169487 Mast_cells EIF4G3 down CD248 2.87E-07 -0.296485116 0.14 0.341 0.015040392 Mast_cells CD248 down IRF2BPL 1.92E-06 -0.296486141 0.233 0.477 0.100433416 Mast_cells IRF2BPL down LPP 2.34E-07 -0.297013024 0.133 0.33 0.012243936 Mast_cells LPP down MLLT1 4.03E-07 -0.297214299 0.182 0.409 0.021112197 Mast_cells MLLT1 down LAMC1 1.13E-08 -0.297512902 0.093 0.284 0.000593135 Mast_cells LAMC1 down QPRT 5.36E-08 -0.298597807 0.193 0.443 0.002804128 Mast_cells QPRT down SSR1 4.68E-07 -0.298671736 0.24 0.489 0.024515354 Mast_cells SSR1 down PPP2R2C 6.71E-10 -0.298865104 0.078 0.273 3.51E-05 Mast_cells PPP2R2C down SLC16A11 3.65E-10 -0.29932708 0.081 0.284 1.91E-05 Mast_cells SLC16A1 down EHD2 8.09E-11 -0.299376621 0.095 0.318 4.24E-06 Mast_cells EHD2 down TIPARP 2.24E-05 -0.2995186 0.421 0.648 1 Mast_cells TIPARP down MYADM 1.52E-06 -0.29963569 0.74 0.909 0.07954045 Mast_cells MYADM down CRISPLD2 3.88E-10 -0.300706236 0.086 0.295 2.03E-05 Mast_cells CRISPLD2 down DPY19L1 9.13E-09 -0.300725466 0.116 0.33 0.00047783 Mast_cells DPY19L1 down LAPTM4B 2.35E-07 -0.300822143 0.202 0.443 0.012307668 Mast_cells LAPTM4B down CANX 5.62E-06 -0.300844045 0.602 0.773 0.294265253 Mast_cells CANX down EIF3A 1.63E-05 -0.301666811 0.425 0.659 0.855413698 Mast_cells EIF3A down RBM5 4.75E-07 -0.301925338 0.22 0.466 0.024854566 Mast_cells RBM5 down NRP1 1.63E-08 -0.302012395 0.143 0.364 0.000851261 Mast_cells NRP1 down LTBP3 1.64E-07 -0.302476773 0.139 0.341 0.008566349 Mast_cells LTBP3 down YWHAG 1.36E-07 -0.303268901 0.248 0.523 0.007114918 Mast_cells YWHAG down ZYG11B 3.96E-12 -0.30328535 0.096 0.341 2.07E-07 Mast_cells ZYG11B down SPON1 1.07E-09 -0.303309377 0.133 0.375 5.61E-05 Mast_cells SPON1 down PLXNB2 6.70E-11 -0.303591254 0.101 0.33 3.51E-06 Mast_cells PLXNB2 down CCNL2 5.44E-07 -0.303963614 0.204 0.432 0.028474145 Mast_cells CCNL2 down PABPN1 3.96E-07 -0.30398641 0.243 0.489 0.020739879 Mast_cells PABPN1 down DDB1 3.78E-07 -0.3041004 0.209 0.443 0.019767958 Mast_cells DDB1 down ADAMTS91 6.28E-12 -0.304221521 0.096 0.341 3.29E-07 Mast_cells ADAMTS9 down CNP 9.52E-12 -0.30480416 0.113 0.375 4.98E-07 Mast_cells CNP down MT-ND5 2.81E-15 -0.30508639 0.971 1 1.47E-10 Mast_cells MT-ND5 down GREB1 1.24E-14 -0.305542227 0.065 0.295 6.47E-10 Mast_cells GREB1 down RPS3AP47 3.56E-14 -0.305711589 0.049 0.261 1.86E-09 Mast_cells RPS3AP47 down MT-TD 1.31E-14 -0.305826725 0.06 0.295 6.84E-10 Mast_cells MT-TD down TIA1 1.41E-08 -0.305901685 0.196 0.466 0.000738515 Mast_cells TIA1 down BASP1 1.57E-05 -0.306171915 0.355 0.58 0.824045884 Mast_cells BASP1 down RBBP6 8.33E-06 -0.3067374 0.375 0.602 0.436054251 Mast_cells RBBP6 down MSL1 5.80E-07 -0.307298748 0.154 0.352 0.030373003 Mast_cells MSL1 down EIF4H 3.82E-06 -0.308895516 0.458 0.716 0.200135614 Mast_cells EIF4H down DST 1.36E-05 -0.309012285 0.227 0.42 0.713623646 Mast_cells DST down SREK1 1.19E-06 -0.309708398 0.254 0.489 0.062550505 Mast_cells SREK1 down TLE5 4.31E-06 -0.309829919 0.444 0.682 0.225713942 Mast_cells TLE5 down SNHG16 4.32E-08 -0.310705137 0.182 0.42 0.002262096 Mast_cells SNHG16 down MEST 5.74E-09 -0.310747713 0.096 0.295 0.000300611 Mast_cells MEST down SERPINE1 2.19E-09 -0.311312565 0.059 0.227 0.000114508 Mast_cells SERPINE1 down PARVA 5.85E-11 -0.311633952 0.117 0.364 3.06E-06 Mast_cells PARVA down WT1 1.64E-10 -0.311902409 0.102 0.33 8.60E-06 Mast_cells WT1 down BCAS1 2.95E-15 -0.312488362 0.052 0.273 1.55E-10 Mast_cells BCAS1 down RAB11B 1.56E-06 -0.313225223 0.304 0.557 0.081923297 Mast_cells RAB11B down ZEB1 7.85E-13 -0.313262517 0.082 0.318 4.11E-08 Mast_cells ZEB1 down IGFBP22 6.95E-05 -0.313581169 0.182 0.352 1 Mast_cells IGFBP2 down COL12A1 2.23E-12 -0.313757098 0.06 0.261 1.17E-07 Mast_cells COL12A1 down TMEM98 7.98E-10 -0.314541633 0.137 0.386 4.18E-05 Mast_cells TMEM98 down REV3L 5.42E-06 -0.315081559 0.284 0.511 0.28359626 Mast_cells REV3L down BMPR2 1.13E-08 -0.31537767 0.159 0.398 0.000593228 Mast_cells BMPR2 down HPSE2 2.18E-19 -0.315822222 0.044 0.284 1.14E-14 Mast_cells HPSE2 down PRDM1 1.58E-12 -0.315825659 0.089 0.33 8.25E-08 Mast_cells PRDM1 down TSHZ3 1.23E-14 -0.316324352 0.078 0.33 6.45E-10 Mast_cells TSHZ3 down HSPB61 1.88E-06 -0.316517841 0.154 0.352 0.098610842 Mast_cells HSPB6 down CS 1.90E-09 -0.317132466 0.144 0.398 9.95E-05 Mast_cells CS down RAB7A 1.25E-06 -0.317272436 0.524 0.773 0.065272664 Mast_cells RAB7A down UBE3A 1.05E-06 -0.31729648 0.275 0.523 0.054953707 Mast_cells UBE3A down SURF4 1.02E-08 -0.318232837 0.246 0.545 0.000536138 Mast_cells SURF4 down SHISA5 1.92E-09 -0.319131247 0.166 0.432 0.000100467 Mast_cells SHISA5 down UBE2R2 1.93E-07 -0.319879723 0.319 0.602 0.010120226 Mast_cells UBE2R2 down KLF10 1.34E-05 -0.320406664 0.452 0.693 0.701922848 Mast_cells KLF10 down ANGPTL2 4.79E-13 -0.320411337 0.071 0.295 2.51E-08 Mast_cells ANGPTL2 down LRP1 7.41E-09 -0.320450579 0.154 0.386 0.000387841 Mast_cells LRP1 down ADAR 2.75E-07 -0.320980293 0.226 0.455 0.014415373 Mast_cells ADAR down ANXA5 1.52E-05 -0.321675612 0.508 0.67 0.79708094 Mast_cells ANXA5 down BRD4 1.17E-07 -0.321824954 0.345 0.659 0.006132349 Mast_cells BRD4 down G6PC3 1.31E-09 -0.322469127 0.138 0.375 6.84E-05 Mast_cells G6PC3 down FLRT2 8.53E-14 -0.322644508 0.083 0.33 4.46E-09 Mast_cells FLRT2 down MLXIP 7.24E-12 -0.323901743 0.131 0.409 3.79E-07 Mast_cells MLXIP down RORB 1.31E-07 -0.324386348 0.15 0.375 0.006847811 Mast_cells RORB down ZKSCAN1 6.21E-08 -0.324774239 0.199 0.443 0.003248929 Mast_cells ZKSCAN1 down NUCKS1 1.34E-07 -0.324843503 0.567 0.83 0.007036 Mast_cells NUCKS1 down PBX1 2.51E-06 -0.325233577 0.491 0.727 0.131296537 Mast_cells PBX1 down DBN1 3.09E-08 -0.325281254 0.165 0.398 0.001618307 Mast_cells DBN1 down LEF11 4.06E-18 -0.325298243 0.062 0.33 2.13E-13 Mast_cells LEF1 down SEMA5A 1.62E-11 -0.325535626 0.078 0.295 8.50E-07 Mast_cells SEMA5A down RBFOX2 1.27E-10 -0.327279936 0.155 0.432 6.64E-06 Mast_cells RBFOX2 down STT3B 1.66E-08 -0.327467646 0.205 0.466 0.000868755 Mast_cells STT3B down GLG1 1.31E-10 -0.328476016 0.137 0.398 6.84E-06 Mast_cells GLG1 down PTBP1 1.47E-06 -0.32853557 0.34 0.602 0.0767664 Mast_cells PTBP1 down CLMP 3.73E-12 -0.329712325 0.114 0.375 1.96E-07 Mast_cells CLMP down GALNT2 6.85E-10 -0.329940615 0.12 0.352 3.58E-05 Mast_cells GALNT2 down CHMP4B 2.90E-07 -0.330170831 0.31 0.591 0.01520107 Mast_cells CHMP4B down NDN 1.91E-07 -0.33063235 0.176 0.398 0.009997697 Mast_cells NDN down IL6ST 1.75E-08 -0.332311924 0.217 0.477 0.000918718 Mast_cells IL6ST down ATF4 2.88E-07 -0.333281503 0.437 0.727 0.01509647 Mast_cells ATF4 down RNF130 6.83E-07 -0.33366062 0.404 0.67 0.035769676 Mast_cells RNF130 down TAB2 4.42E-09 -0.334767505 0.177 0.443 0.00023167 Mast_cells TAB2 down TMED10 7.49E-07 -0.33504948 0.447 0.625 0.039227126 Mast_cells TMED10 down EMP1 3.70E-12 -0.335363342 0.069 0.284 1.94E-07 Mast_cells EMP1 down CHST14 5.31E-14 -0.336014757 0.09 0.352 2.78E-09 Mast_cells CHST14 down IGFBP7 1.74E-05 -0.336127208 0.343 0.568 0.910910803 Mast_cells IGFBP7 down ERGIC1 2.93E-10 -0.336348353 0.212 0.523 1.53E-05 Mast_cells ERGIC1 down AKT2 3.94E-09 -0.336995025 0.149 0.386 0.000206433 Mast_cells AKT2 down LENG8 1.39E-08 -0.338490499 0.225 0.511 0.00072914 Mast_cells LENG8 down RPL34P27 3.78E-14 -0.340402735 0.095 0.375 1.98E-09 Mast_cells RPL34P27 down TPP1 4.94E-07 -0.340501377 0.301 0.568 0.025845475 Mast_cells TPP1 down SND1 7.94E-09 -0.340591503 0.219 0.489 0.000415533 Mast_cells SND1 down ACO₂ 2.05E-09 -0.340963396 0.179 0.443 0.000107222 Mast_cells ACO₂ down SCARB2 2.99E-09 -0.342531627 0.171 0.42 0.000156474 Mast_cells SCARB2 down LOXL2 1.27E-12 -0.344067264 0.073 0.295 6.66E-08 Mast_cells LOXL2 down YWHAE 1.54E-09 -0.344789478 0.649 0.875 8.06E-05 Mast_cells YWHAE down DDR2 8.54E-10 -0.345692819 0.135 0.375 4.47E-05 Mast_cells DDR2 down RPL7P9 1.41E-12 -0.345886099 0.106 0.375 7.40E-08 Mast_cells RPL7P9 down ZBTB7A 1.27E-07 -0.346497946 0.303 0.568 0.006661604 Mast_cells ZBTB7A down AL590867.2 6.65E-14 -0.346554542 0.084 0.341 3.48E-09 Mast_cells AL590867.2 down AMOTL2 5.50E-16 -0.346732472 0.064 0.307 2.88E-11 Mast_cells AMOTL2 down GANAB 2.83E-08 -0.346842979 0.201 0.443 0.001481643 Mast_cells GANAB down SHFL 9.62E-09 -0.34685627 0.176 0.42 0.000503588 Mast_cells SHFL down PDGFRA 2.51E-13 -0.347088835 0.112 0.386 1.32E-08 Mast_cells PDGFRA down HDAC7 5.67E-13 -0.34736614 0.107 0.375 2.97E-08 Mast_cells HDAC7 down SPTLC2 1.00E-10 -0.347910173 0.138 0.398 5.25E-06 Mast_cells SPTLC2 down ATXN1 1.96E-08 -0.348995006 0.26 0.557 0.001023623 Mast_cells ATXN1 down PCBP2 8.54E-08 -0.349089684 0.535 0.761 0.004473202 Mast_cells PCBP2 down CALU 8.93E-07 -0.349203475 0.355 0.602 0.046751726 Mast_cells CALU down SRF 1.16E-10 -0.349825829 0.113 0.352 6.09E-06 Mast_cells SRF down ADAM121 1.60E-06 -0.350055035 0.245 0.455 0.08401966 Mast_cells ADAM12 down OLFM1 7.74E-12 -0.350296998 0.101 0.341 4.05E-07 Mast_cells OLFM1 down SSBP3 6.62E-07 -0.351121598 0.289 0.534 0.034642468 Mast_cells SSBP3 down SMARCA2 6.74E-08 -0.351482822 0.246 0.511 0.003527851 Mast_cells SMARCA2 down ZFP362 4.49E-08 -0.35335048 0.76 0.898 0.002350554 Mast_cells ZFP36 down GPX8 1.92E-12 -0.354057752 0.125 0.398 1.00E-07 Mast_cells GPX8 down MT-ND4L 1.93E-13 -0.354275161 0.855 0.977 1.01E-08 Mast_cells MT-ND4L down ATXN2L 2.42E-08 -0.354329928 0.238 0.511 0.001268824 Mast_cells ATXN2L down RPL31 1.57E-16 -0.354575224 0.894 0.989 8.24E-12 Mast_cells RPL31 down IL321 1.21E-12 -0.354875433 0.096 0.352 6.36E-08 Mast_cells IL32 down SULF2 3.29E-10 -0.356338363 0.151 0.409 1.72E-05 Mast_cells SULF2 down ATN1 2.07E-08 -0.357224301 0.239 0.523 0.001081385 Mast_cells ATN1 down PRRX1 2.56E-11 -0.358676922 0.148 0.432 1.34E-06 Mast_cells PRRX1 down PRRC2B 1.15E-09 -0.358845823 0.215 0.5 6.04E-05 Mast_cells PRRC2B down HAND2-AS1 5.39E-15 -0.359568632 0.111 0.409 2.82E-10 Mast_cells HAND2-AS1 down ESR1 8.57E-10 -0.359618558 0.188 0.466 4.49E-05 Mast_cells ESR1 down WNT2 3.12E-13 -0.360176262 0.104 0.364 1.63E-08 Mast_cells WNT2 down KDELR1 1.37E-07 -0.360465202 0.443 0.716 0.007187458 Mast_cells KDELR1 down RBP12 9.45E-07 -0.36103698 0.189 0.398 0.049460953 Mast_cells RBP1 down MXRA7 1.60E-11 -0.361105303 0.152 0.432 8.39E-07 Mast_cells MXRA7 down RAB5B 1.36E-12 -0.361235756 0.166 0.489 7.15E-08 Mast_cells RAB5B down PLOD1 8.02E-12 -0.363081378 0.138 0.409 4.20E-07 Mast_cells PLOD1 down FOXL2 1.91E-13 -0.364403977 0.097 0.352 1.00E-08 Mast_cells FOXL2 down PPIC 1.49E-12 -0.365013646 0.12 0.386 7.80E-08 Mast_cells PPIC down ERRFI1 1.54E-13 -0.365151905 0.074 0.307 8.05E-09 Mast_cells ERRFI1 down LAMB1 3.36E-10 -0.368215587 0.106 0.33 1.76E-05 Mast_cells LAMB1 down MTRNR2L12 1.39E-14 -0.369403664 0.118 0.432 7.28E-10 Mast_cells MTRNR2L12 down AL031727.1 2.28E-16 -0.370555462 0.067 0.33 1.20E-11 Mast_cells AL031727.1 down MAP4 1.34E-08 -0.370627619 0.251 0.511 0.000701682 Mast_cells MAP4 down HIC1 1.00E-09 -0.370694469 0.162 0.42 5.26E-05 Mast_cells HIC1 down CAPNS1 3.73E-08 -0.372256936 0.433 0.705 0.001951432 Mast_cells CAPNS1 down PENK 1.59E-13 -0.372992575 0.049 0.25 8.34E-09 Mast_cells PENK down TRIB1 1.25E-10 -0.373354409 0.103 0.33 6.56E-06 Mast_cells TRIB1 down SYNCRIP 3.90E-09 -0.373372491 0.335 0.648 0.000204076 Mast_cells SYNCRIP down PMEPA1 1.08E-06 -0.373507637 0.392 0.614 0.056604476 Mast_cells PMEPA1 down MTCO1P12 2.20E-18 -0.373571169 0.076 0.375 1.15E-13 Mast_cells MTCO1P12 down ZNF516 8.85E-12 -0.373696057 0.134 0.398 4.63E-07 Mast_cells ZNF516 down CRTAP 2.45E-07 -0.373750622 0.379 0.625 0.012847767 Mast_cells CRTAP down PAMR1 6.20E-10 -0.373759184 0.126 0.364 3.25E-05 Mast_cells PAMR1 down MFAP2 3.71E-11 -0.374351619 0.147 0.42 1.94E-06 Mast_cells MFAP2 down COL27A1 7.99E-10 -0.374422147 0.137 0.375 4.19E-05 Mast_cells COL27A1 down BTBD2 5.45E-15 -0.374711084 0.113 0.409 2.86E-10 Mast_cells BTBD2 down RCN1 5.41E-09 -0.375096373 0.209 0.466 0.000283401 Mast_cells RCN1 down SSR3 1.13E-07 -0.375312384 0.449 0.716 0.005940912 Mast_cells SSR3 down BEX3 1.59E-08 -0.375326439 0.244 0.523 0.000832733 Mast_cells BEX3 down H1F0 7.20E-10 -0.375558086 0.214 0.511 3.77E-05 Mast_cells H1F0 down AC116533.1 3.16E-13 -0.37850405 0.14 0.455 1.66E-08 Mast_cells AC116533.1 down FSCN1 2.36E-10 -0.378979637 0.171 0.443 1.24E-05 Mast_cells FSCN1 down DAB2 8.70E-09 -0.380337304 0.205 0.455 0.000455503 Mast_cells DAB2 down SLC38A2 8.44E-08 -0.3816798 0.323 0.58 0.004418513 Mast_cells SLC38A2 down GJA1 8.88E-09 -0.38239662 0.157 0.386 0.000465186 Mast_cells GJA1 down CLIC4 4.51E-13 -0.382659419 0.148 0.443 2.36E-08 Mast_cells CLIC4 down CDC42EP4 8.52E-10 -0.384169086 0.161 0.409 4.46E-05 Mast_cells CDC42EP4 down LTBP1 4.38E-13 -0.384284826 0.137 0.432 2.29E-08 Mast_cells LTBP1 down MYL9 6.05E-09 -0.384653845 0.186 0.443 0.00031672 Mast_cells MYL9 down U2AF2 7.00E-11 -0.385824961 0.249 0.591 3.66E-06 Mast_cells U2AF2 down SPIN1 1.13E-10 -0.386182764 0.232 0.545 5.93E-06 Mast_cells SPIN1 down CREB3L1 3.12E-13 -0.386940659 0.104 0.364 1.63E-08 Mast_cells CREB3L1 down CHSY1 6.30E-13 -0.38712777 0.144 0.443 3.30E-08 Mast_cells CHSY1 down OSR2 2.61E-09 -0.391110431 0.168 0.42 0.000136433 Mast_cells OSR2 down IMPAD1 2.39E-12 -0.391306669 0.14 0.42 1.25E-07 Mast_cells IMPAD1 down AC243919.1 1.18E-19 -0.39147637 0.056 0.33 6.16E-15 Mast_cells AC243919.1 down CNN2 1.19E-10 -0.393593975 0.296 0.67 6.22E-06 Mast_cells CNN2 down ITGB1 6.90E-08 -0.394800538 0.404 0.659 0.003615075 Mast_cells ITGB1 down OS9 2.38E-09 -0.39625149 0.352 0.659 0.000124479 Mast_cells OS9 down TPM4 4.44E-10 -0.397052776 0.612 0.795 2.33E-05 Mast_cells TPM4 down KPNB1 2.21E-09 -0.397570333 0.3 0.591 0.000115586 Mast_cells KPNB1 down GASK1B 8.82E-13 -0.398302534 0.124 0.398 4.62E-08 Mast_cells GASK1B down SH3PXD2A 9.74E-12 -0.399436532 0.187 0.5 5.10E-07 Mast_cells SH3PXD2A down ITGA6 1.05E-12 -0.399798474 0.108 0.364 5.51E-08 Mast_cells ITGA6 down RAB31 1.33E-12 -0.400817124 0.119 0.386 6.99E-08 Mast_cells RAB31 down PTCH1 2.87E-11 -0.404380698 0.168 0.455 1.50E-06 Mast_cells PTCH1 down C1S 2.58E-11 -0.404495871 0.168 0.455 1.35E-06 Mast_cells C1S down RAI14 1.56E-13 -0.404989708 0.141 0.443 8.17E-09 Mast_cells RAI14 down SDK11 4.84E-17 -0.407884572 0.091 0.386 2.53E-12 Mast_cells SDK1 down WNT5A1 6.35E-15 -0.408623349 0.094 0.364 3.32E-10 Mast_cells WNT5A down FLNA 3.10E-10 -0.410831007 0.283 0.602 1.62E-05 Mast_cells FLNA down MME 9.41E-16 -0.411974366 0.094 0.375 4.93E-11 Mast_cells MME down PLPP1 4.30E-09 -0.412456521 0.294 0.591 0.000225403 Mast_cells PLPP1 down IFI61 5.53E-10 -0.412668985 0.246 0.557 2.90E-05 Mast_cells IFI6 down APCDD12 9.89E-12 -0.412889267 0.116 0.364 5.18E-07 Mast_cells APCDD1 down CKAP4 2.65E-10 -0.413201109 0.184 0.455 1.39E-05 Mast_cells CKAP4 down HNRNPUL1 4.43E-11 -0.41320559 0.289 0.648 2.32E-06 Mast_cells HNRNPUL1 down TCF12 5.09E-13 -0.413912844 0.162 0.466 2.67E-08 Mast_cells TCF12 down TBX3 1.08E-14 -0.414275321 0.145 0.466 5.64E-10 Mast_cells TBX3 down RPL6P27 3.08E-17 -0.414984229 0.087 0.386 1.61E-12 Mast_cells RPL6P27 down ANXA2 3.07E-10 -0.416724411 0.244 0.534 1.61E-05 Mast_cells ANXA2 down CALD1 5.87E-10 -0.419429411 0.234 0.534 3.07E-05 Mast_cells CALD1 down EIF4G2 9.80E-13 -0.420170415 0.597 0.875 5.13E-08 Mast_cells EIF4G2 down EWSR1 1.16E-10 -0.42045889 0.306 0.636 6.10E-06 Mast_cells EWSR1 down RYBP 8.78E-10 -0.42075844 0.377 0.716 4.60E-05 Mast_cells RYBP down RPL37P6 5.57E-25 -0.421546105 0.05 0.352 2.92E-20 Mast_cells RPL37P6 down KLF42 2.24E-08 -0.421612196 0.403 0.693 0.001172165 Mast_cells KLF4 down MARCKSL1 3.38E-08 -0.422074904 0.356 0.614 0.001770014 Mast_cells MARCKSL1 down HDLBP 1.01E-09 -0.422773776 0.383 0.67 5.31E-05 Mast_cells HDLBP down PRSS231 2.74E-10 -0.422855285 0.192 0.477 1.43E-05 Mast_cells PRSS23 down FBLN11 6.97E-07 -0.423471564 0.332 0.557 0.036509022 Mast_cells FBLN1 down PSAP 5.26E-14 -0.423503977 0.714 0.955 2.75E-09 Mast_cells PSAP down MYH9 7.07E-10 -0.4251328 0.326 0.625 3.70E-05 Mast_cells MYH9 down ATP2A2 5.15E-11 -0.426758232 0.282 0.625 2.69E-06 Mast_cells ATP2A2 down COL7A1 5.74E-12 -0.427602002 0.13 0.398 3.01E-07 Mast_cells COL7A1 down CDK2AP1 1.45E-10 -0.427930668 0.272 0.58 7.59E-06 Mast_cells CDK2AP1 down TUG1 1.16E-12 -0.430135499 0.213 0.545 6.08E-08 Mast_cells TUG1 down VCL 3.92E-13 -0.431433868 0.19 0.511 2.05E-08 Mast_cells VCL down PTMS 2.62E-09 -0.435906189 0.493 0.693 0.000137425 Mast_cells PTMS down GLIPR11 4.09E-14 -0.439354137 0.098 0.364 2.14E-09 Mast_cells GLIPR1 down TTC3 5.07E-10 -0.442967483 0.345 0.648 2.66E-05 Mast_cells TTC3 down ZNF704 3.73E-11 -0.446403249 0.188 0.466 1.95E-06 Mast_cells ZNF704 down SOCS32 2.80E-09 -0.447068775 0.217 0.5 0.000146771 Mast_cells SOCS3 down LUM 3.69E-10 -0.451819808 0.215 0.511 1.93E-05 Mast_cells LUM down CCND2 6.21E-11 -0.452794497 0.295 0.625 3.25E-06 Mast_cells CCND2 down RPL3P4 1.63E-19 -0.45331721 0.107 0.455 8.52E-15 Mast_cells RPL3P4 down MAGED1 1.52E-12 -0.454141795 0.181 0.489 7.94E-08 Mast_cells MAGED1 down RPL26P191 1.61E-16 -0.454212882 0.143 0.5 8.44E-12 Mast_cells RPL26P19 down GNB1 3.26E-11 -0.45588357 0.422 0.773 1.71E-06 Mast_cells GNB1 down MIR1244-2 6.79E-15 -0.456071731 0.171 0.534 3.56E-10 Mast_cells MIR1244-2 down ANTXR1 4.00E-17 -0.456361731 0.106 0.409 2.10E-12 Mast_cells ANTXR1 down PCSK51 7.29E-16 -0.456984653 0.111 0.409 3.82E-11 Mast_cells PCSK5 down F13A11 2.27E-18 -0.457918671 0.06 0.318 1.19E-13 Mast_cells F13A1 down CALR 3.21E-14 -0.460081843 0.634 0.898 1.68E-09 Mast_cells CALR down CTDSP2 2.86E-15 -0.460581735 0.165 0.5 1.50E-10 Mast_cells CTDSP2 down MAP3K20 6.23E-12 -0.460756581 0.176 0.466 3.26E-07 Mast_cells MAP3K20 down TCF4 4.89E-11 -0.462113148 0.211 0.511 2.56E-06 Mast_cells TCF4 down TWIST2 8.37E-14 -0.463067401 0.137 0.432 4.38E-09 Mast_cells TWIST2 down SEC31A 2.49E-12 -0.466461782 0.341 0.705 1.31E-07 Mast_cells SEC31A down NID1 1.19E-17 -0.471191323 0.123 0.455 6.24E-13 Mast_cells NID1 down HOXA10 5.23E-15 -0.471925409 0.197 0.568 2.74E-10 Mast_cells HOXA10 down RPL15P3 8.71E-20 -0.472945706 0.081 0.398 4.56E-15 Mast_cells RPL15P3 down MARCKS1 5.13E-11 -0.474755159 0.216 0.511 2.69E-06 Mast_cells MARCKS down CAVIN1 2.76E-11 -0.480110495 0.381 0.682 1.44E-06 Mast_cells CAVIN1 down FBN1 1.43E-17 -0.480715421 0.137 0.477 7.50E-13 Mast_cells FBN1 down IGFBP4 5.36E-09 -0.481978028 0.253 0.511 0.00028074 Mast_cells IGFBP4 down UBA1 7.58E-16 -0.482341846 0.237 0.648 3.97E-11 Mast_cells UBA1 down NFIC 4.12E-11 -0.483249205 0.321 0.636 2.15E-06 Mast_cells NFIC down AC007969.1 2.28E-23 -0.484167766 0.08 0.432 1.19E-18 Mast_cells AC007969.1 down PGR1 3.09E-13 -0.484317096 0.202 0.523 1.62E-08 Mast_cells PGR down HDGF 2.36E-14 -0.485448057 0.234 0.591 1.24E-09 Mast_cells HDGF down GDF7 1.54E-15 -0.485570594 0.138 0.455 8.08E-11 Mast_cells GDF7 down ZFP36L1 1.80E-10 -0.485747347 0.527 0.795 9.44E-06 Mast_cells ZFP36L1 down PXDN 1.59E-18 -0.488633271 0.111 0.432 8.31E-14 Mast_cells PXDN down PIM1 4.58E-10 -0.490331074 0.203 0.466 2.40E-05 Mast_cells PIM1 down PALLD 3.58E-11 -0.492113715 0.301 0.625 1.88E-06 Mast_cells PALLD down MMP14 3.57E-14 -0.492133751 0.162 0.489 1.87E-09 Mast_cells MMP14 down LGALS3BP 1.39E-11 -0.492746362 0.203 0.489 7.26E-07 Mast_cells LGALS3BP down FN11 1.06E-15 -0.496490959 0.156 0.5 5.55E-11 Mast_cells FN1 down KHSRP 2.08E-14 -0.500966221 0.207 0.557 1.09E-09 Mast_cells KHSRP down AL022718.1 1.58E-26 -0.501600698 0.063 0.409 8.26E-22 Mast_cells AL022718.1 down MATR31 1.58E-15 -0.502042549 0.193 0.591 8.29E-11 Mast_cells MATR3 down SIGLEC17P 2.08E-14 -0.504760421 0.237 0.659 1.09E-09 Mast_cells SIGLEC17P down APP 6.24E-13 -0.506488117 0.194 0.5 3.27E-08 Mast_cells APP down COL4A2 1.58E-15 -0.506999148 0.185 0.534 8.27E-11 Mast_cells COL4A2 down MGP3 1.12E-07 -0.509942039 0.227 0.455 0.005883726 Mast_cells MGP down SDC2 8.22E-18 -0.517253838 0.121 0.443 4.30E-13 Mast_cells SDC2 down SPARCL1 3.57E-10 -0.523477532 0.268 0.568 1.87E-05 Mast_cells SPARCL1 down USP22 2.09E-15 -0.530823006 0.26 0.636 1.09E-10 Mast_cells USP22 down RTL8C 1.59E-16 -0.532589912 0.232 0.625 8.31E-12 Mast_cells RTL8C down VCAN3 2.71E-14 -0.537710535 0.182 0.523 1.42E-09 Mast_cells VCAN down C11orf961 3.72E-10 -0.54005638 0.219 0.5 1.95E-05 Mast_cells C11orf96 down H19 1.15E-11 -0.543136334 0.19 0.477 6.01E-07 Mast_cells H19 down C1R 7.09E-17 -0.544896424 0.177 0.534 3.71E-12 Mast_cells C1R down CDH11 5.38E-16 -0.545938183 0.187 0.534 2.82E-11 Mast_cells CDH11 down EDNRA 1.32E-14 -0.547365225 0.163 0.477 6.91E-10 Mast_cells EDNRA down COL14A1 3.41E-15 -0.550272961 0.174 0.511 1.79E-10 Mast_cells COL14A1 down OLFML3 3.08E-17 -0.55031253 0.172 0.534 1.61E-12 Mast_cells OLFML3 down CNN31 1.61E-15 -0.553082636 0.182 0.523 8.44E-11 Mast_cells CNN3 down PCOLCE 1.28E-13 -0.554112516 0.231 0.568 6.73E-09 Mast_cells PCOLCE down COL6A2 6.85E-14 -0.556188384 0.248 0.591 3.58E-09 Mast_cells COL6A2 down RHOB1 3.82E-10 -0.557721653 0.384 0.659 2.00E-05 Mast_cells RHOB down OAF 8.57E-15 -0.558228242 0.279 0.636 4.48E-10 Mast_cells OAF down MDK 3.57E-10 -0.563285726 0.345 0.614 1.87E-05 Mast_cells MDK down ZYX 3.00E-13 -0.566670296 0.272 0.58 1.57E-08 Mast_cells ZYX down IRF2BP2 1.28E-14 -0.56723429 0.43 0.784 6.70E-10 Mast_cells IRF2BP2 down PGRMC1 3.37E-13 -0.576261851 0.288 0.625 1.77E-08 Mast_cells PGRMC1 down TGFBI2 2.07E-22 -0.589986194 0.136 0.534 1.09E-17 Mast_cells TGFBI down THY1 2.31E-13 -0.590183305 0.22 0.545 1.21E-08 Mast_cells THY1 down IGFBP51 2.87E-08 -0.595356569 0.301 0.545 0.001502045 Mast_cells IGFBP5 down FSTL1 3.78E-18 -0.599326619 0.193 0.568 1.98E-13 Mast_cells FSTL1 down MMP2 1.88E-17 -0.606538957 0.205 0.602 9.85E-13 Mast_cells MMP2 down DCN1 4.60E-16 -0.606896297 0.206 0.557 2.41E-11 Mast_cells DCN down COL6A3 5.81E-19 -0.608836447 0.18 0.568 3.04E-14 Mast_cells COL6A3 down ALDH1A2 5.82E-17 -0.61504784 0.164 0.5 3.05E-12 Mast_cells ALDH1A2 down AP001324.1 4.66E-34 -0.617194159 0.069 0.477 2.44E-29 Mast_cells AP001324.1 down CPXM1 1.71E-18 -0.629520731 0.199 0.58 8.94E-14 Mast_cells CPXM1 down MTND1P231 4.84E-20 -0.639203871 0.163 0.568 2.53E-15 Mast_cells MTND1P23 down SPON2 3.16E-19 -0.640974581 0.187 0.568 1.66E-14 Mast_cells SPON2 down CD241 1.01E-16 -0.647022843 0.202 0.557 5.28E-12 Mast_cells CD24 down SFRP1 4.10E-13 -0.658303825 0.26 0.591 2.15E-08 Mast_cells SFRP1 down TIMP2 4.28E-19 -0.669105286 0.212 0.602 2.24E-14 Mast_cells TIMP2 down NR2F2 6.36E-16 -0.671889043 0.259 0.625 3.33E-11 Mast_cells NR2F2 down CRABP2 9.85E-21 -0.674472549 0.165 0.557 5.15E-16 Mast_cells CRABP2 down SERPINH11 1.04E-17 -0.675029777 0.21 0.58 5.45E-13 Mast_cells SERPINH1 down ISLR 1.11E-18 -0.698169855 0.196 0.58 5.80E-14 Mast_cells ISLR down COL5A1 1.13E-15 -0.703550909 0.239 0.58 5.90E-11 Mast_cells COL5A1 down ECM14 4.04E-21 -0.703863952 0.181 0.591 2.11E-16 Mast_cells ECM1 down COL5A2 1.22E-22 -0.705125465 0.167 0.58 6.40E-18 Mast_cells COL5A2 down OGN1 1.09E-21 -0.736951553 0.153 0.534 5.68E-17 Mast_cells OGN down DIO23 2.00E-17 -0.737543797 0.187 0.557 1.05E-12 Mast_cells DIO2 down MT-ATP81 5.13E-20 -0.74156612 0.391 0.807 2.68E-15 Mast_cells MT-ATP8 down COL6A11 2.22E-18 -0.748873874 0.26 0.648 1.16E-13 Mast_cells COL6A1 down MMP111 2.12E-15 -0.760899733 0.239 0.58 1.11E-10 Mast_cells MMP11 down SFRP43 3.36E-12 -0.763150146 0.501 0.739 1.76E-07 Mast_cells SFRP4 down NREP2 1.24E-23 -0.788150734 0.223 0.659 6.49E-19 Mast_cells NREP down COL1A2 5.84E-17 -0.802074847 0.432 0.784 3.06E-12 Mast_cells COL1A2 down SPARC1 8.06E-16 -0.823842149 0.334 0.67 4.22E-11 Mast_cells SPARC down IGF11 5.98E-18 -0.829965275 0.268 0.648 3.13E-13 Mast_cells IGF1 down MTATP6P12 4.32E-20 -0.834021712 0.304 0.795 2.26E-15 Mast_cells MTATP6P1 down MT-RNR12 1.95E-19 -0.87648487 0.315 0.807 1.02E-14 Mast_cells MT-RNR1 down COL1A11 3.53E-19 -0.904405832 0.514 0.864 1.85E-14 Mast_cells COL1A1 down COL3A13 1.51E-24 -0.962218181 0.485 0.92 7.93E-20 Mast_cells COL3A1 down MT-RNR22 4.01E-17 -0.966083104 0.315 0.807 2.10E-12 Mast_cells MT-RNR2 down CCND11 1.90E-271 0.640852415 0.548 0.111 9.96E-267 mit_Fibro CCND1 up IGFBP61 2.93E-126 0.585931727 0.46 0.181 1.53E-121 mit_Fibro IGFBP6 up PLAC91 1.03E-110 0.442078187 0.439 0.182 5.40E-106 mit_Fibro PLAC9 up CDKN1A2 3.39E-86 0.387207999 0.6 0.327 1.78E-81 mit_Fibro CDKN1A up SCGB2A16 9.26E-187 0.378774039 0.465 0.106 4.85E-182 mit_Fibro SCGB2A1 up GDF152 1.79E-99 0.353809496 0.367 0.12 9.38E-95 mit_Fibro GDF15 up WFDC26 6.44E-81 0.317228785 0.461 0.227 3.37E-76 mit_Fibro WFDC2 up ANXA1 1.30E-67 0.317085663 0.662 0.433 6.79E-63 mit_Fibro ANXA1 up BGN 1.19E-65 0.310270399 0.615 0.43 6.25E-61 mit_Fibro BGN up HSPB62 1.99E-57 0.300658956 0.753 0.586 1.04E-52 mit_Fibro HSPB6 up SOCS33 2.26E-29 0.298793306 0.673 0.549 1.18E-24 mit_Fibro SOCS3 up APOE1 4.10E-40 0.292414269 0.741 0.642 2.14E-35 mit_Fibro APOE up HMGA11 1.01E-52 0.287781674 0.267 0.108 5.30E-48 mit_Fibro HMGA1 up GPNMB1 5.36E-72 0.274568625 0.549 0.309 2.81E-67 mit_Fibro GPNMB up NKAIN41 9.38E-190 0.271485544 0.263 0.009 4.91E-185 mit_Fibro NKAIN4 up COL18A11 5.16E-67 0.266274288 0.506 0.277 2.70E-62 mit_Fibro COL18A1 up ZMAT31 3.20E-66 0.265299376 0.507 0.273 1.68E-61 mit_Fibro ZMAT3 up LOXL1 5.76E-72 0.262089667 0.523 0.29 3.02E-67 mit_Fibro LOXL1 up RPS27L 2.74E-68 0.259614738 0.814 0.703 1.44E-63 mit_Fibro RPS27L up TIMP32 5.27E-54 0.258515729 0.711 0.512 2.76E-49 mit_Fibro TIMP3 up FXYD61 1.76E-65 0.257862426 0.54 0.318 9.22E-61 mit_Fibro FXYD6 up S100A42 1.65E-58 0.25407323 0.424 0.222 8.65E-54 mit_Fibro S100A4 up CLU3 7.18E-46 0.250544984 0.57 0.407 3.76E-41 mit_Fibro CLU up S100A61 5.77E-61 0.250057249 0.865 0.796 3.02E-56 mit_Fibro S100A6 up OGN2 2.08E-45 -0.261412445 0.594 0.762 1.09E-40 mit_Fibro OGN down IL322 7.99E-44 -0.27052503 0.389 0.565 4.19E-39 mit_Fibro IL32 down F13A12 3.30E-52 -0.277377718 0.509 0.698 1.73E-47 mit_Fibro F13A1 down TNC2 6.29E-49 -0.289040527 0.501 0.665 3.29E-44 mit_Fibro TNC down SCGB2A17 8.30E-269 0.662104335 0.413 0.058 4.35E-264 NK_cells SCGB2A1 up WFDC27 4.52E-210 0.648777682 0.468 0.142 2.37E-205 NK_cells WFDC2 up MT2A2 2.40E-31 0.432363158 0.412 0.315 1.26E-26 NK_cells MT2A up MT1X2 6.21E-39 0.421247452 0.274 0.161 3.25E-34 NK_cells MT1X up CXCR4 1.42E-109 0.414073179 0.723 0.504 7.43E-105 NK_cells CXCR4 up SCGB1D22 4.58E-126 0.350011596 0.225 0.033 2.40E-121 NK_cells SCGB1D2 up IGFBP52 1.79E-42 0.345443534 0.361 0.227 9.35E-38 NK_cells IGFBP5 up ITM2C 1.96E-65 0.333252465 0.493 0.311 1.03E-60 NK_cells ITM2C up CLU4 1.94E-77 0.324685996 0.256 0.095 1.02E-72 NK_cells CLU up LMNA1 3.86E-48 0.284757955 0.488 0.353 2.02E-43 NK_cells LMNA up GNG2 3.70E-50 0.273219457 0.351 0.202 1.94E-45 NK_cells GNG2 up CAPS 2.62E-74 0.271154674 0.245 0.086 1.37E-69 NK_cells CAPS up FBLN12 5.33E-31 0.265329371 0.413 0.31 2.79E-26 NK_cells FBLN1 up EZR 1.34E-69 0.264204238 0.788 0.671 7.04E-65 NK_cells EZR up PAEP1 3.63E-91 0.251920764 0.124 0.004 1.90E-86 NK_cells PAEP up SYTL3 7.88E-47 -0.250320936 0.307 0.474 4.13E-42 NK_cells SYTL3 down RBP13 4.61E-39 -0.251522089 0.214 0.342 2.41E-34 NK_cells RBP1 down TMIGD2 3.93E-49 -0.253194603 0.316 0.485 2.06E-44 NK_cells TMIGD2 down DAPK2 7.36E-65 -0.254983767 0.122 0.289 3.85E-60 NK_cells DAPK2 down CD38 1.30E-56 -0.263410087 0.283 0.475 6.81E-52 NK_cells CD38 down RNF1301 1.26E-65 -0.268117873 0.246 0.428 6.61E-61 NK_cells RNF130 down LINC00996 1.23E-74 -0.276035438 0.116 0.294 6.42E-70 NK_cells LINC00996 down CMC1 3.23E-58 -0.281631464 0.514 0.678 1.69E-53 NK_cells CMC1 down FCER1G1 8.36E-83 -0.289587848 0.623 0.783 4.38E-78 NK_cells FCER1G down TRDC 4.99E-79 -0.292019339 0.607 0.759 2.61E-74 NK_cells TRDC down NCAM1 1.93E-65 -0.293767781 0.345 0.559 1.01E-60 NK_cells NCAM1 down KIR2DL3 2.32E-83 -0.293875704 0.079 0.249 1.22E-78 NK_cells KIR2DL3 down CCL3 2.67E-38 -0.295896978 0.427 0.552 1.40E-33 NK_cells CCL3 down EPAS1 3.20E-67 -0.300311821 0.276 0.471 1.67E-62 NK_cells EPAS1 down KIR2DL4 1.99E-79 -0.3199576 0.153 0.345 1.04E-74 NK_cells KIR2DL4 down ECM15 1.28E-56 -0.339918195 0.19 0.339 6.69E-52 NK_cells ECM1 down ITGAX 5.10E-83 -0.348461095 0.246 0.481 2.67E-78 NK_cells ITGAX down COTL1 3.70E-114 -0.379873696 0.603 0.803 1.94E-109 NK_cells COTL1 down GZMB 4.28E-118 -0.499115035 0.344 0.607 2.24E-113 NK_cells GZMB down GNLY2 2.28E-152 -0.62962152 0.463 0.713 1.19E-147 NK_cells GNLY down ADIRF1 6.84E-80 0.571275386 0.711 0.336 3.58E-75 SMC ADIRF up PTGDS 1.35E-44 0.563886143 0.532 0.303 7.09E-40 SMC PTGDS up MT2A3 3.36E-72 0.540267635 0.846 0.634 1.76E-67 SMC MT2A up BTG22 4.16E-85 0.522278884 0.799 0.514 2.18E-80 SMC BTG2 up ATF32 2.95E-77 0.510354775 0.834 0.639 1.54E-72 SMC ATF3 up CKB1 9.11E-82 0.509568703 0.803 0.535 4.77E-77 SMC CKB up MT1E1 9.02E-60 0.48769541 0.473 0.191 4.72E-55 SMC MT1E up COL14A11 2.07E-74 0.478870208 0.766 0.463 1.08E-69 SMC COL14A1 up SOCS34 2.52E-72 0.477925822 0.835 0.603 1.32E-67 SMC SOCS3 up CDKN1A3 1.81E-66 0.457471854 0.603 0.282 9.47E-62 SMC CDKN1A up CFD1 4.49E-71 0.447770979 0.353 0.061 2.35E-66 SMC CFD up FXYD1 8.51E-69 0.441094846 0.615 0.291 4.46E-64 SMC FXYD1 up EPHA7 5.17E-64 0.438708269 0.35 0.077 2.71E-59 SMC EPHA7 up PTN1 7.07E-52 0.428351124 0.657 0.403 3.70E-47 SMC PTN up KLF22 1.43E-59 0.426711316 0.659 0.384 7.50E-55 SMC KLF2 up S100A62 1.10E-51 0.416189173 0.799 0.632 5.75E-47 SMC S100A6 up S100A43 5.96E-44 0.415643354 0.68 0.442 3.12E-39 SMC S100A4 up CCL22 3.15E-34 0.410835218 0.436 0.217 1.65E-29 SMC CCL2 up NR4A11 2.64E-50 0.406349503 0.782 0.578 1.38E-45 SMC NR4A1 up SCGB2A18 2.17E-82 0.401415315 0.453 0.108 1.14E-77 SMC SCGB2A1 up HSPA1B2 1.38E-49 0.398991715 0.771 0.521 7.23E-45 SMC HSPA1B up TGM22 8.56E-44 0.392587256 0.532 0.299 4.48E-39 SMC TGM2 up DNAJB12 2.91E-65 0.392244079 0.879 0.758 1.52E-60 SMC DNAJB1 up WFDC28 1.75E-59 0.376394405 0.532 0.229 9.18E-55 SMC WFDC2 up PLAC92 5.20E-45 0.372885903 0.655 0.436 2.72E-40 SMC PLAC9 up MFAP4 1.37E-66 0.365309358 0.372 0.083 7.16E-62 SMC MFAP4 up HSPA1A2 7.78E-53 0.358084568 0.906 0.788 4.07E-48 SMC HSPA1A up GADD45B1 1.36E-38 0.357106328 0.799 0.66 7.12E-34 SMC GADD45B up MYC 6.47E-41 0.353428142 0.681 0.459 3.39E-36 SMC MYC up EPAS11 6.26E-51 0.352881861 0.734 0.483 3.28E-46 SMC EPAS1 up CEBPD 2.25E-45 0.348218621 0.814 0.697 1.18E-40 SMC CEBPD up IRF11 4.71E-43 0.346183013 0.811 0.665 2.47E-38 SMC IRF1 up MAFF2 2.36E-45 0.341293016 0.73 0.527 1.24E-40 SMC MAFF up GAS6 1.51E-54 0.340789747 0.774 0.562 7.89E-50 SMC GAS6 up MSX12 2.83E-50 0.335525216 0.465 0.19 1.48E-45 SMC MSX1 up ZFP363 2.04E-44 0.334752191 0.865 0.749 1.07E-39 SMC ZFP36 up FOSB1 1.85E-55 0.334169298 0.93 0.817 9.70E-51 SMC FOSB up MUSTN1 2.82E-30 0.332145954 0.314 0.127 1.47E-25 SMC MUSTN1 up TIPARP1 3.62E-35 0.331450163 0.584 0.376 1.90E-30 SMC TIPARP up SLIT3 5.25E-43 0.328996973 0.689 0.409 2.75E-38 SMC SLIT3 up DUSP11 2.58E-43 0.324989653 0.796 0.665 1.35E-38 SMC DUSP1 up CLU5 2.52E-67 0.324493586 0.358 0.072 1.32E-62 SMC CLU up HSPB63 3.83E-38 0.315996751 0.53 0.292 2.01E-33 SMC HSPB6 up RGS16 8.48E-29 0.314252735 0.727 0.553 4.44E-24 SMC RGS16 up CRISPLD21 1.42E-36 0.312899056 0.703 0.507 7.45E-32 SMC CRISPLD2 up LITAF 7.69E-45 0.309429412 0.751 0.572 4.02E-40 SMC LITAF up KLF43 2.44E-40 0.305510094 0.447 0.205 1.28E-35 SMC KLF4 up CD92 5.74E-41 0.305397315 0.316 0.102 3.00E-36 SMC CD9 up SERTAD11 4.73E-40 0.301567618 0.739 0.604 2.48E-35 SMC SERTAD1 up MT1X3 3.57E-26 0.292013719 0.322 0.152 1.87E-21 SMC MT1X up SPARCL11 4.54E-49 0.291434337 0.914 0.838 2.37E-44 SMC SPARCL1 up PGF1 2.56E-32 0.290668328 0.608 0.397 1.34E-27 SMC PGF up ECRG4 1.43E-53 0.288828694 0.307 0.065 7.47E-49 SMC ECRG4 up ADAMTS1 6.37E-27 0.284391249 0.661 0.491 3.34E-22 SMC ADAMTS1 up ANXA11 4.76E-36 0.281738941 0.419 0.197 2.49E-31 SMC ANXA1 up EFEMP11 5.32E-43 0.277039345 0.277 0.068 2.79E-38 SMC EFEMP1 up TOB1 3.24E-29 0.275678125 0.561 0.373 1.69E-24 SMC TOB1 up ID3 3.55E-27 0.275432948 0.761 0.609 1.86E-22 SMC ID3 up RARRES21 1.35E-31 0.274324736 0.735 0.598 7.07E-27 SMC RARRES2 up JUNB1 2.99E-59 0.274272944 0.934 0.895 1.57E-54 SMC JUNB up DPEP11 1.67E-45 0.273460817 0.328 0.095 8.74E-41 SMC DPEP1 up MYH11 3.86E-18 0.27283983 0.515 0.346 2.02E-13 SMC MYH11 up C11orf962 5.28E-49 0.267469206 0.96 0.901 2.76E-44 SMC C11orf96 up ID2 1.10E-26 0.267082339 0.5 0.31 5.76E-22 SMC ID2 up MIR99AHG 4.38E-28 0.262978661 0.581 0.379 2.29E-23 SMC MIR99AHG up NFIL3 1.76E-27 0.262692149 0.575 0.397 9.21E-23 SMC NFIL3 up NR4A3 1.36E-39 0.261609887 0.325 0.111 7.13E-35 SMC NR4A3 up TIMP33 5.88E-35 0.252353569 0.905 0.802 3.08E-30 SMC TIMP3 up EGR12 2.54E-35 0.251643984 0.919 0.845 1.33E-30 SMC EGR1 up TWIST1 2.16E-27 -0.250166284 0.266 0.442 1.13E-22 SMC TWIST1 down SOX4 2.65E-67 -0.250453397 0.92 0.979 1.39E-62 SMC SOX4 down ANGPT2 1.87E-21 -0.252332065 0.514 0.685 9.82E-17 SMC ANGPT2 down MIR4435- 2HG 4.80E-26 -0.252834904 0.572 0.726 2.51E-21 SMC MIR4435- 2HG down SPON21 4.74E-30 -0.254186389 0.725 0.832 2.48E-25 SMC SPON2 down EGFL6 1.03E-25 -0.254235791 0.289 0.462 5.39E-21 SMC EGFL6 down LINGO1 1.67E-30 -0.255892648 0.333 0.548 8.74E-26 SMC LINGO1 down FN12 7.39E-33 -0.257010664 0.746 0.862 3.87E-28 SMC FN1 down MARCKSL11 9.66E-45 -0.258128485 0.77 0.886 5.06E-40 SMC MARCKSL1 down GLIPR12 1.35E-28 -0.259295226 0.246 0.424 7.08E-24 SMC GLIPR1 down STMN1 2.22E-27 -0.261117263 0.503 0.666 1.16E-22 SMC STMN1 down TBX31 1.32E-30 -0.261534547 0.581 0.721 6.92E-26 SMC TBX3 down C4orf482 3.93E-33 -0.262657413 0.424 0.606 2.06E-28 SMC C4orf48 down HOPX 1.79E-16 -0.262824982 0.439 0.554 9.36E-12 SMC HOPX down ENC1 6.85E-26 -0.263299807 0.407 0.581 3.59E-21 SMC ENC1 down FARP1 2.05E-36 -0.264612171 0.575 0.752 1.08E-31 SMC FARP1 down COL4A11 2.96E-65 -0.264676098 0.932 0.988 1.55E-60 SMC COL4A1 down FAP 2.35E-29 -0.269423762 0.259 0.434 1.23E-24 SMC FAP down PXDN1 9.30E-40 -0.271495837 0.631 0.809 4.87E-35 SMC PXDN down TSHZ2 6.00E-32 -0.274145657 0.359 0.593 3.14E-27 SMC TSHZ2 down AGPAT5 2.21E-36 -0.276446062 0.309 0.518 1.16E-31 SMC AGPAT5 down WNT5A2 1.25E-30 -0.27815796 0.167 0.344 6.53E-26 SMC WNT5A down FBLIM1 3.99E-43 -0.280640845 0.639 0.821 2.09E-38 SMC FBLIM1 down FAM162B 3.19E-24 -0.281238077 0.326 0.491 1.67E-19 SMC FAM162B down PLAT 1.08E-31 -0.281650056 0.543 0.699 5.67E-27 SMC PLAT down LOXL21 1.40E-25 -0.283139491 0.405 0.56 7.32E-21 SMC LOXL2 down NES 5.90E-36 -0.283265546 0.681 0.797 3.09E-31 SMC NES down ADAM122 7.18E-42 -0.285015484 0.232 0.481 3.76E-37 SMC ADAM12 down PELI1 2.19E-34 -0.287195023 0.415 0.606 1.15E-29 SMC PELI1 down CASC151 1.08E-38 -0.289169378 0.309 0.531 5.66E-34 SMC CASC15 down BMP11 8.05E-46 -0.293007801 0.533 0.733 4.21E-41 SMC BMP1 down HTRA3 3.97E-27 -0.295043426 0.185 0.347 2.08E-22 SMC HTRA3 down DIO24 1.02E-28 -0.295696596 0.538 0.708 5.35E-24 SMC DIO2 down RGS4 3.72E-29 -0.296395403 0.243 0.432 1.95E-24 SMC RGS4 down LAMB11 1.58E-41 -0.298404395 0.634 0.821 8.26E-37 SMC LAMB1 down A2M 2.89E-46 -0.302951106 0.707 0.85 1.51E-41 SMC A2M down COL5A21 1.28E-60 -0.303766932 0.804 0.932 6.68E-56 SMC COL5A2 down CD2481 1.69E-53 -0.305016477 0.727 0.868 8.84E-49 SMC CD248 down RARRES1 2.43E-42 -0.313734068 0.143 0.359 1.27E-37 SMC RARRES1 down CYTOR 1.69E-52 -0.355140932 0.687 0.85 8.85E-48 SMC CYTOR down MARCKS2 1.50E-87 -0.355863421 0.816 0.939 7.87E-83 SMC MARCKS down SULF1 6.96E-49 -0.375054107 0.423 0.661 3.64E-44 SMC SULF1 down FJX11 4.31E-52 -0.380900567 0.504 0.742 2.25E-47 SMC FJX1 down MXRA5 1.50E-73 -0.433106589 0.258 0.577 7.83E-69 SMC MXRA5 down MMP112 2.01E-83 -0.436462422 0.765 0.926 1.05E-78 SMC MMP11 down ECM16 4.47E-83 -0.470454782 0.525 0.807 2.34E-78 SMC ECM1 down APCDD13 1.75E-75 -0.473679016 0.441 0.735 9.15E-71 SMC APCDD1 down NREP3 1.60E-118 -0.494715598 0.707 0.944 8.36E-114 SMC NREP down RBP14 2.84E-117 -0.529063378 0.61 0.886 1.49E-112 SMC RBP1 down PAEP2 5.44E-265 1.1623452 0.62 0.159 2.85E-260 unciliated_EpC PAEP up CLU6 2.70E-231 0.983878697 0.696 0.352 1.41E-226 unciliated_EpC CLU up SOD31 5.80E-167 0.688610558 0.549 0.225 3.04E-162 unciliated_EpC SOD3 up SLPI 2.26E-177 0.650635762 0.821 0.606 1.19E-172 unciliated_EpC SLPI up PIGR1 6.43E-149 0.641209842 0.617 0.34 3.37E-144 unciliated_EpC PIGR up SCGB1D23 9.21E-140 0.611770699 0.808 0.62 4.82E-135 unciliated_EpC SCGB1D2 up CCDC146 3.27E-166 0.607075352 0.666 0.377 1.71E-161 unciliated_EpC CCDC146 up SCGB2A19 1.56E-285 0.571232212 0.972 0.846 8.16E-281 unciliated_EpC SCGB2A1 up SCGB3A1 1.28E-107 0.519548912 0.43 0.155 6.73E-103 unciliated_EpC SCGB3A1 up KLK11 9.28E-122 0.510322135 0.495 0.221 4.86E-117 unciliated_EpC KLK11 up HLA-DRB11 8.32E-66 0.44446909 0.413 0.231 4.35E-61 unciliated_EpC HLA-DRB1 up SRARP 9.29E-111 0.437326736 0.403 0.128 4.86E-106 unciliated_EpC SRARP up CP1 1.15E-81 0.41324638 0.327 0.104 6.04E-77 unciliated_EpC CP up HLA-DRA1 2.95E-62 0.405326558 0.486 0.317 1.54E-57 unciliated_EpC HLA-DRA up TFF32 4.36E-91 0.388112355 0.341 0.098 2.28E-86 unciliated_EpC TFF3 up FBLN13 1.27E-122 0.375490301 0.835 0.79 6.65E-118 unciliated_EpC FBLN1 up CST1 9.27E-53 0.35628316 0.232 0.073 4.86E-48 unciliated_EpC CST1 up IL1R1 1.60E-70 0.335352832 0.615 0.457 8.38E-66 unciliated_EpC IL1R1 up IGFBP71 4.95E-56 0.333531545 0.691 0.599 2.59E-51 unciliated_EpC IGFBP7 up CST4 6.63E-73 0.330990252 0.197 0.019 3.47E-68 unciliated_EpC CST4 up IFI62 5.46E-55 0.326903828 0.655 0.529 2.86E-50 unciliated_EpC IFI6 up CREB3L11 3.43E-56 0.309080962 0.564 0.359 1.80E-51 unciliated_EpC CREB3L1 up EHF 2.47E-68 0.307712225 0.717 0.591 1.29E-63 unciliated_EpC EHF up GNG11 3.31E-63 0.307017183 0.672 0.542 1.73E-58 unciliated_EpC GNG11 up SERPINA5 2.94E-53 0.302573283 0.688 0.593 1.54E-48 unciliated_EpC SERPINA5 up NUPR1 4.31E-49 0.302144161 0.418 0.248 2.26E-44 unciliated_EpC NUPR1 up PALMD 3.09E-50 0.302033406 0.404 0.239 1.62E-45 unciliated_EpC PALMD up SERPING1 4.59E-51 0.299544156 0.492 0.353 2.40E-46 unciliated_EpC SERPING1 up CKB2 8.17E-48 0.292096787 0.683 0.56 4.28E-43 unciliated_EpC CKB up TCEA3 8.43E-63 0.284903249 0.418 0.215 4.41E-58 unciliated_EpC TCEA3 up IGFBP62 5.76E-36 0.284298298 0.218 0.094 3.01E-31 unciliated_EpC IGFBP6 up TXNIP1 6.67E-87 0.276058784 0.886 0.793 3.49E-82 unciliated_EpC TXNIP up MPC2 2.22E-47 0.269947773 0.586 0.505 1.16E-42 unciliated_EpC MPC2 up CD74 3.24E-43 0.265365212 0.706 0.676 1.70E-38 unciliated_EpC CD74 up CFH 1.20E-39 0.263708202 0.271 0.134 6.31E-35 unciliated_EpC CFH up PHLDA3 3.95E-47 0.257367761 0.345 0.188 2.07E-42 unciliated_EpC PHLDA3 up ACSL5 1.07E-27 0.255155907 0.565 0.494 5.59E-23 unciliated_EpC ACSL5 up RXFP1 1.38E-48 0.254568254 0.374 0.205 7.22E-44 unciliated_EpC RXFP1 up HLA-DMA 3.50E-29 0.252405565 0.416 0.323 1.83E-24 unciliated_EpC HLA-DMA up PKHD1L11 1.39E-40 0.251097817 0.448 0.284 7.26E-36 unciliated_EpC PKHD1L1 up SFRP44 9.24E-35 -0.250606461 0.662 0.809 4.84E-30 unciliated_EpC SFRP4 down CCN2 2.56E-39 -0.260096957 0.388 0.547 1.34E-34 unciliated_EpC CCN2 down AC246817.1 2.68E-157 -0.26609397 0.068 0.303 1.41E-152 unciliated_EpC AC246817.1 down EMID1 2.73E-63 -0.268517634 0.384 0.582 1.43E-58 unciliated_EpC EMID1 down TRMT9B 3.01E-75 -0.26930473 0.235 0.45 1.58E-70 unciliated_EpC TRMT9B down NSG1 4.46E-113 -0.270043691 0.111 0.329 2.33E-108 unciliated_EpC NSG1 down COL3A14 1.25E-39 -0.278328584 0.618 0.797 6.52E-35 unciliated_EpC COL3A1 down C4orf483 4.35E-57 -0.285959093 0.436 0.594 2.28E-52 unciliated_EpC C4orf48 down MSX2 2.90E-82 -0.290349308 0.341 0.604 1.52E-77 unciliated_EpC MSX2 down ECM17 1.00E-57 -0.292500053 0.196 0.376 5.24E-53 unciliated_EpC ECM1 down VCAN4 1.02E-66 -0.304145317 0.347 0.576 5.34E-62 unciliated_EpC VCAN down NREP4 1.37E-79 -0.315798204 0.276 0.508 7.18E-75 unciliated_EpC NREP down GLA 3.21E-83 -0.324268946 0.196 0.403 1.68E-78 unciliated_EpC GLA down GCLC 2.56E-81 -0.3335096 0.399 0.606 1.34E-76 unciliated_EpC GCLC down MGP4 5.62E-78 -0.336425559 0.263 0.498 2.94E-73 unciliated_EpC MGP down LGALS13 6.24E-82 -0.353952597 0.571 0.809 3.26E-77 unciliated_EpC LGALS1 down CPM 7.77E-97 -0.36113717 0.695 0.856 4.07E-92 unciliated_EpC CPM down RBP15 1.84E-103 -0.38041321 0.329 0.598 9.63E-99 unciliated_EpC RBP1 down PDLIM11 4.27E-134 -0.41185447 0.591 0.791 2.23E-129 unciliated_EpC PDLIM1 down IGFBP23 2.14E-139 -0.435023616 0.338 0.664 1.12E-134 unciliated_EpC IGFBP2 down Figure 2.

Bulk RNA-seq Profiles in EP versus EU, Aberrant Gene Expression in Cell Types from Endometrial Polyps’ samples. (A) The PCA plot displays the distribution of individuals along the first two principal components (Dim1 and Dim2). Individuals are colorcoded by group: EP (blue circles) and EU (orange triangles). (B) The volcano plot displays gene expression changes from EP to EU of bulk RNA-seq (EU, n = 5; EP, n = 5), highlighting significant genes (green) versus non-significant ones (red) based on log2 fold change and adjusted p-values. (C) Bar plots illustrating the gene ontology (GO) enrichment analysis of genes that are either upregulated or downregulated between EU and EP. Fisher’s exact tests (two-sided) were conducted, with a p.adjust of less than 0.05 considered statistically significant.

(D-E) Dot plots showing the relative expression changes of specific genes from bulk RNA-seq data across various cell types in single-cell analyses. The size of the dots indicates the percentage of expression. Endometrial polyps exhibit cell type-specific changes in their transcriptional regulatory programs To distinguish between the shared and distinct gene expression alterations linked to endometrial polyps within each cell type, we examined the differentially expressed genes (DEGs) across the major cell types found in both endometrial (EU) and polyp samples. A total of 1,534 DEGs associated with polyps (with |log2FoldChange| > 0.25 and adjusted P value < 0.05) were identified, exhibiting differential expression in at least one cell type across both EU and MP samples (Fig.

3A), Major cell types in endometrium, included Mast cell, SMC, ciliated-epithelia and unciliated-epithelia, were most affected by polpys, as manifested by high DEG counts of 727, 236, 124 and 120 respectively (Fig 4B and Table S2), While there was significant variability in the differentially expressed genes (DEGs) among various cell types, certain Gene Ontology (GO) term annotations were common across the primary cell types. For example, the downregulated DEGs were enriched in biological regulation process (ADAM12, APCDD1, BMP1, and COL3A1) and Upregulated DEGs were associated with inflammatory response and antigen processing (MSX1, ATF3, CLU, and IRF1), metabolic process (NME2, NDUFA7, and, LDHB), and response factor cellular such as: response to hormone (AR, JAK1/3, CRY1, STAT3, FOS) (Fig 3B).

Furthermore, Estrogen is implicated in the development of endometrial polyps, and a detailed analysis indicated an increased expression of genes associated with the estrogen response, as identified in the Molecular Signatures Database (MSigDB) (Fig.3C), In a joint analysis of differentially expressed genes (DEGs) within the estrogen response database, the gene set score (AUCell) of whole tissue and multiple cell types demonstrated an activation of genes related to estrogen response, except for mast cells and unciliated epithelia (Fig.3C and Fig.S3). Consistently, we identified that the majority of genes involved in the estrogen response were upregulated across multiple cell types, except for mast cells (Fig.3D). Notably, TGM2, a gene associated with extracellular matrix remodeling and apoptosis16 , exhibited elevated expression levels in fibroblasts and smooth muscle cells (SMCs).

Additionally, TFF3, which possesses anti-apoptotic properties17 , and CKB, involved in cellular energy homeostasis18 , were both found to be increased in the epithelial cells of endometrial polyps. Conversely, the hormone receptors AR (Androgen Receptor) and PGR (Progesterone Receptor) were downregulated in mast cells within endometrial polyps (Fig.3E). Next, we performed SCENIC analysis to identify key transcription factors (TFs) present in various cell types within endometrial polyps. (Fig. 3F and 3G). The identified core TFs include FOSB, FOS, JUNB, and JUND, which are components of the AP-1 complex that showed upregulation in epithelial cells, macrophages, endothelial cells, and SMCs. These factors are associated with cell proliferation, apoptosis, and stress responses, and their expression changes have also been observed in colorectal polyps19 .

Furthermore, KLF6, KLF2, and KLF4, members of the KLF family, were found to be upregulated in fibroblasts, endothelial cells, and SMCs. These factors are crucial for cell proliferation, with KLF4 notably promoting the formation of intestinal polyps20 . EGR1, which has been noted for altered expression in patients with nasal polyps, was also upregulated in the endothelial cells and SMCs of endometrial polyps. Additionally, we observed the downregulation of HOXA10, a gene implicated in endometrial polyps 21 , particularly in mast cells, suggesting a distinctive transcriptional network within these cells (Fig.3G). Notably, the analysis of transcription factors (TFs) that were co-downregulated across different cell types indicated that mast cell transcription factors were the most significantly downregulated.

Taken together, these results shed light on the transcriptional regulatory mechanisms underlying endometrial polyps, identifying crucial factors that drive their development and progression. Figure 3. Transcriptional changes in various cell types between EU and EP. (A) Circular diagram displays gene expression changes in endometrial polyps across various cell types. significant downregulated genes are shown in blue (sigDown), while upregulated genes are in red (sigUp). The placement of dots represent expression levels, offering insights into differential gene expression among cell populations. (B) Network illustrates the representative Gene Ontology (GO) terms and pathways associated with differentially expressed genes (DEGs) between endometrial uterine (EU) and endometrial polyp (EP) samples. Nodes are depicted as pie charts, with the size of each pie reflecting the total number of genes associated with that term.

The pie charts are color-coded by cell types, and the size of each slice indicates the proportion of genes from the specific gene list contributing to that term. Connections between two terms are drawn if their similarity exceeds 0.3. (C) Ridge plot showing the AUC score of estrogen response gene set in EP. (D) Network visualization highlights differentially expressed genes (DEGs) that are upregulated (in red) and downregulated (on the right) between endometrial uterine (EU) and endometrial polyp samples, specifically those that overlap with genes associated with estrogen response gene sets. The pie charts on the left depict various cell types, with red indicating upregulated genes and blue representing downregulated ones.

The size of each node reflects the number of target genes linked to a particular cell type. Red nodes signify upregulated DEGs, while the blue nodes on the right denote downregulated DEGs. (E) Network plot illustrates the upregulated and downregulated differentially expressed genes (DEGs) across all cell types that intersect with genes annotated in the estrogen response gene sets. The size of each node reflects the occurrence of DEGs across various cell types. The red sections of the nodes represent upregulated genes, while the blue sections indicate downregulated genes. (F) Heatmap displaying the SCENIC analysis of cell type-specific core regulatory transcription factors (TFs) in polyps. (G) Network visualization depicts the core regulatory transcription factors (TFs) that are upregulated (on the left) and downregulated (on the right) in endometrial polyps.

The pie charts in the center illustrate various cell types, with red indicating upregulated TFs and green indicating downregulated ones. The left side showcases red nodes representing upregulated TFs, while the right side features blue nodes for downregulated TFs. Increase in Mast Cell Number and Activation Associated with Susceptibility to Endometrial Polyp Development To gain a deeper understanding of the transcriptomic landscape of endometrial polyps, we utilized augur analysis. This method identifies cell types that are most responsive to biological perturbations in single-cell data22 . Our findings indicated that mast cells were the most significantly affected cell type in endometrial polyps (Fig.4A). The changes in cell proportions and transcriptional profiles suggested that mast cells play a pivotal role in the development of endometrial polyps (Fig.1H and 4B).

Mast cells contribute to inflammation and tissue remodeling within polyps by releasing cytokines and growth factors, thereby affecting the growth and persistence of these polyps23-25 . The tryptase serves as a marker of mast-cell activation26 , and our analysis revealed significant activation of mast cells within endometrial polyps (Fig.4C). To investigate this, we performed gene enrichment analysis of DEGs in mast cells. The results showed significant enrichment of upregulated genes also associated with granulocyte activation (CTSG, TYROBP, IL18) and proton motive force-driven ATP synthesis (ATP6V0C, NDUFA13, ATP5F1D). Conversely, downregulated genes were enriched in pathways related to gland morphogenesis (MMP2, IGFBP5, MDK) and female gonad development (NR2F2, SFRP1, PGR) (Fig.4D). Next, we explored whether cellular communications between mast cells and other cell types in the EPs were altered.

Utilizing the bioinformatic tool CellphoneDB, we assessed enhanced cell-cell communications in the EPs based on upregulated differentially expressed genes. Our analysis revealed that steroid-related signaling, specifically the interaction of 5alphaDihydroprogesterone facilitated by DHRS9 with the progesterone receptor (PGR), and tumor necrosis factor signaling through TNF-TNFRSF1B interactions, were enhanced between mast cells and epithelial/fibroblast cells in the EPs. This finding aligns with previous studies showing significantly higher levels of PGR in glandular epithelium in EPs27 . Furthermore, angiogenesis signaling, particularly the VEGFA- NRP2 interaction, demonstrated increased activity between mast cells and endothelial cells (Fig.4E). These findings suggest that the increase and activation of mast cells in the EPs are closely correlated with a heightened susceptibility to the development of endometrial polyps.

Transcription factor (TF) network analysis identified 15 specific regulators with significant differences between EPs and EU. Notably, the WT1 regulon was found to be downregulated in endometrial polyps and central to a gene network that influences mast cell function (Fig.4F). Both the transcription level and regulon activity of WT1 were decreased in endometrial polyps and verified downregulation of WT1 in EPs mast cells by immunofluorescence (Fig.4H and 4C). Sequencing data indicated that WT1 broadly regulates 37 differentially expressed genes (DEGs) in mast cells, with these target genes showing disturbances in endometrial polyps as WT1 expression decreases (Fig.4Jand 4H). Specifically, the DEGs targeted by WT1 were linked to cell growth (WT1, IGF1, and WNT2) and epithelial to mesenchymal transition (SFRP1, WNT2, WNT5A) (Fig 4I).

These findings imply that the repression of WT1 and the disruption of its downstream transcriptional network likely contribute to the proliferation of mast cells in endometrial polyps. Figure 4. Profiling of mast cell changes in endometrial polyps. (A) Circular plot illustrates the prioritization of cell types that are most reactive to biological changes during the formation of endometrial polyps. This analysis was conducted using Augur, a technique designed to identify cell types exhibiting the most significant responses in singlecell data. (B) Rose diagram illustrates the counts of differentially expressed genes (DEGs) in various cell types from endometrial polyp formation. Upregulated DEGs are represented in red, while downregulated DEGs are shown in green (C) Co-staining of Tryptase and WT1 in EU and EP.

Left, representative image of Tryptase and WT1 in EU and EP. Middle, the relative Tryptase positive cells was quantiffed. Right, quantitative data of WT1 expression in Mast cells. Scale bars, 10μm and 5μm (zoomed-in images). **P < 0.01. (D) Bar plot displays the significance of biological processes (BP) associated with differentially expressed genes, Upregulated DEGs are represented in red, while downregulated DEGs are shown in blue. The x-axis represents the negative logarithm of the p-values. (E) Visualization of cell-cell communication interactions identified using CellPhoneDB. The dot plot displays the mean interaction scores between various cell types, specifically focusing on interactions involving mast cells and other cell populations. The size of each dot corresponds to the mean interaction score.

The color gradient represents the mean scores, with purple denoting lower values and green indicating higher values. A red dot indicates statistically significant interactions. Interactions are listed on the y-axis, while the different cell types are represented on the x-axis. (E) Regulatory network illustrates potential key transcriptional regulators in mast cells. In this visualization, larger nodes signify transcription factors (TFs), while smaller nodes represent target genes. The size of the TF nodes is directly proportional to the number of genes they regulate. Nodes colored in red indicate upregulation, whereas blue nodes denote downregulation. (F) Figure presents the expression of various transcription factors (TFs) in mast cell across EP and EU.

The upper section displays violin plots showing the expression of each TF in mast cell across EP and EU. The lower section features a heatmap representing the regulation activity of each TF in mast cell across EP and EU. the size of the black circles below indicates the number of target genes associated with each TF. (G) A ridge plot displays the AUC scores of WT1-regulated gene sets in polyps. (I) Network plot illustrates the enrichment of representative terms associated with WT1 target genes. The genes linked to these terms are displayed on the outer edge of the diagram. Knockdown of WT1 promote Mast cell proliferation in endometrial polyps To explore the functional role of WT1 in endometrial polyps, WT1 was knocked down in the human mast cell line HMC-1 using siRNA.

Post-transfection, WT1 reduction was confirmed at both RNA and protein levels via RT-qPCR and western blot analyses (Fig.5A and 5B). Following WT1 knockdown, HMC-1 cell proliferation was significantly enhanced (Fig.5C). RNA sequencing was subsequently conducted to evaluate gene expression changes. Volcano plot analysis revealed significant upregulation and downregulation of various genes after WT1 knockdown (Fig.5D), indicating its regulatory role in mast cell function.KEGG pathway analysis highlighted modifications in crucial signaling pathways, such as the estrogen signaling pathway and central carbon metabolism, relevant to cell growth and proliferation (Fig 5E). Furthermore, Gene Ontology (GO) enrichment analysis showed that knocking down WT1 affected pathways involved in lipid metabolism (e.g., PLPP2, ALOX5, and ECH1), ribosome biogenesis (e.g., NOP9, LYAR, WDR46), and telomere maintenance (e.g., TERT, PPP1R10, PINX1) (Fig.5F and 5G).

Notably, several differentially expressed genes (DEGs) were found to overlap between WT1-knockdown HMC-1 cells and EPs mast cells. This overlap included no upregulated genes but featured five downregulated genes that are significantly associated with the proliferation and apoptosis of HMC-1 (Fig.5H). Collectively, these findings provide a molecular overview of how WT1 deficiency enhances mast cell proliferation. This underscores WT1’s essential role in managing pathways linked to cellular growth and metabolism, suggesting that its disruption may disturb cellular equilibrium and contribute to conditions like endometrial polyps. Figure 5. Knockdown of WT1 induced Mast cell proliferation in endometrial polyps. (A) qPCR results show the efficiency of WT1 knockdown. *P < 0.05, **P < 0.01.

(B )Westernblot show the efficiency of WT1 knockdown. (C) Cell number demonstrate cell proliferation after WT1 knockdown. (D) Volcano plot showing differentially expressed genes in HMC-1 mast cells following WT1 knockdown. Genes with significant upregulation are marked in red, while those with significant downregulation are marked in blue. (E) KEGG pathway analysis identifying altered signaling pathways, including the estrogen signaling pathway and central carbon metabolism. (F-G) GO enrichment analysis of biological processes affected by WT1 knockdown (H) Venn diagram illustrates the genes that are common between the differentially expressed genes (DEGs) identified in HMC-1 cells following WT1 silencing and those found in mast cells through snRNA-seq analysis. (I) Schematic (produced using Figdraw) illustrates the pathological alterations observed in endometrial polyps.

Discussion

Inthisstudy,weperformedsingle-cellRNAsequencing(scRNAseq) to construct a comprehensive cellular and molecular atlas of endometrial polyps and adjacent eutopic endometrium (EU) tissues. Our findings provide significant insights into the cellular composition changes, gene expression profiles, and transcriptional regulatory mechanisms associated with EPs, with a particular emphasis on the role of mast cells and the transcription factor WT1. The scRNA-seq analysis revealed substantial shifts in cell populations within EPs compared to EU tissues. Specifically, there was an increase in the proportions of epithelial cells, mast cells, and macrophages, along with a decrease in fibroblasts. Immunohistochemistry staining confirmed these observations, showing elevated numbers of CD68-positive macrophages and TPSB2-positive mast cells in the polyps. These findings suggest that glandular proliferation and immune cell infiltration are key features of EPs, contributing to their proliferative and inflammatory characteristics (Fig.5I).

Transcription factor analysis revealed crucial regulatory elements, including the AP-1 complex (FOSB, FOS, JUNB, and JUND) and KLF family members (KLF6, KLF2, and KLF4), which were upregulated in various cell types within EPs. These transcription factors are associated with key processes such as cell proliferation, apoptosis, and stress responses, aligning with the pathological features of EPs. Additionally, the downregulation of HOXA10 and other transcription factors in mast cells indicated a unique transcriptional network in these cells, underscoring their significance in EPs biology. Mast cell proliferation is a key factor in nasal polyp hyperplasia during type II inflammation14. Similarly, we observed a high density of activated mast cells in endometrial polyps, aligning with previous studies15.

This suggests a strong connection between mast cell proliferation and endometrial polyps. WT1, initially identified as a tumor suppressor in Wilms’ Tumor, influences various cancers and developmental processes through target genes like IGF1R, PDGFA, EGF, and TGF-β. Our research indicates a significant reduction of WT1 expression in mast cells within endometrial polyps. Experiments with WT1 knockdown in the HMC-1 mast cell line showed that decreased WT1 levels lead to increased mast cell proliferation and activity, highlighting WT1’s suppressive role. This aligns with prior findings linking c-KIT (CD117) receptor mutations to mast cell proliferation, contributing to conditions such as systemic mastocytosis. Previously, the mechanisms behind mast cell proliferation in uterine polyps were unclear.

Our study addresses this by demonstrating that WT1 downregulation disrupts its regulatory network, resulting in abnormal mast cell growth in endometrial polyps. In conclusion, our research indicates that the downregulation of WT1 in mast cells is a crucial factor driving their proliferation and the inflammatory milieu in endometrial polyps. Targeting WT1 and its related regulatory networks could provide new therapeutic avenues for treating endometrial polyps. Limitations of the study Despite the significant findings regarding the cellular and molecular landscape of endometrial polyps presented in this study, the sample size, although sufficient for exploratory analyses, was limited to seven patients, which may affect the generalizability of the results. Larger, more diverse cohorts are needed to validate the findings and assess potential variations in different populations.

Additionally, the functional assays performed, such as the WT1 knockdown in the HMC-1 mast cell line, may not completely replicate the in vivo conditions present in endometrial polyps. It is crucial to conduct further experiments in primary human mast cells or relevant animal models to confirm the functional relevance of the identified transcriptional changes. Resource availability Lead contact Information and requests for resources should be directed to the lead contact, Hong Xu ([email protected]) or Xiaoying He ([email protected]) Materials availability This study did not generate new unique reagents. Data and code availability The accession number for the single-cell sequencing data reported in this paper is deposited in the China National Center for Bioinformation under the accession number PRJCA032064.

Methods

All methods can be found in the accompanying transparent methods supplemental file. Acknowledgments We would like to express our sincere gratitude to Professor Lihui Lin for providing the HMC-1 cell line, which significantly contributed to the success of this study. This work was supported by the National Natural Science Foundation of China [NO. 8247168, 82271679, 82071622, XH]. Author contributions H.X.Y and H.X. ,Y.L. conceptualized the study and supervised the project; Y.Y., Y.W.Q., W.Z.S., H.T, and L.C conducted the experiments; Y.L performed the bioinformatic analyses of scRNA-seq and RNA-seq; T.H., and C.L., collected clinical samples; Y.L., H.X.Y and H.X wrote the manuscript, with all the authors contributing to writing and providing feedback.

Declaration of interests The authors declare no competing interests.

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Single-Cell Sequencing Reveals a Regulatory Role of WT1 in Mast Cell Proliferation in Endometrial Polyps

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